ﻓﺼﻠﻨﺎﻣﻪ ﺗﻨﺪرﺳﺘﻲ و داﻧﺶ، دوره5 ﺷﻤﺎره، 2 و3 ، ﭘﺎﻳﻴﺰ و ﺗﺎﺑﺴﺘﺎن1389 ﭘﺰﺷﻜ ﻋﻠﻮم داﻧﺸﮕﺎه ﺑﻬﺪاﺷﺘﻲ ﺧﺪﻣﺎت و ﻲ ﺷﺎﻫﺮود درﻣﺎﻧﻲ ﻣﺴ ﻧﻮﻳﺴﻨﺪه ﻮ ول: ﺗﻬﺮان- ﺗﻬﺮان ﭘﺰﺷﻜﻲ ﻋﻠﻮم داﻧﺸﮕﺎه- ﺑﻬﺪاﺷﺖ داﻧﺸﻜﺪه- ﺣﺸﺮه ﮔﺮوه ﻣﺪﻳﺮ و اﺳﺘﺎد و ﭘﺰﺷﻜﻲ ﺷﻨﺎﺳﻲ ﻧﺎﻗﻠﻴﻦ ﺑﺎ ﻣﺒﺎرزه، ﺗﻠﻔﻦ: 88951393 - 021 ، E-mail: rassiy@sina.tums.ac.ir ﭘﮋوﻫﺸﻲ ﻣﻘﺎﻟﻪ ﺟﺪاﺳﺎزي ﺗﻮراﻧﻴﻜﺎ ﻟﻴﺸﻤﺎﻧﻴﺎ اﻧﮕﻞ ﻓﻠﺒﻮﺗﻮﻣﻮس از ﺑ ﭘﺎﭘﺎﺗﺎﺳﻲ ﻪ وﺳﻴﻠﻪ ﺗﻌﻴﻴﻦ ﺗﻮاﻟﻲ ﻧﺎﺣﻴﻪ(Internal Transcribed Spacer 1) ITS1 ﺳﻤﻨﺎن اﺳﺘﺎن داﻣﻐﺎن، ﺷﻬﺮﺳﺘﺎن روﺳﺘﺎﻳﻲ ﺟﻠﺪي ﻟﻴﺸﻤﺎﻧﻴﻮز ﻛﺎﻧﻮن در ازﻧﻲ ﻣﺤﻤﺪي ﺻﺎدق 1 ) M.Sc. ( ، راﺛﻲ ﻳﺎور 2 * ) Ph.D. ( ، ﻋﺸﺎﻗﻲ ﻣﺤﻤﺪﻋﻠﻲ 3 ) Ph.D. ( ، ارﺷﺎدي ﻳﻌﻘﻮﺑﻲ ﻣﺤﻤﺪرﺿﺎ 4 ) Ph.D. ( ، ﻣﺤﺒﻌﻠ ﻣﻬﺪي ﻲ 2 ) Ph.D. ( ، ﻣﺤﻤﺪرﺿﺎ ﻋﺒﺎﻳﻲ 5 ) M.Sc. ( ، ﻣﺤﺘﺮﻣﻲ ﻓﺎﻃﻤﻪ 5 ) M.Sc. ( ، ﻧﻮﻛﻨﺪه زﻳﻨﺐ 6 ) B.Sc. ( ، رﻓﻴﻊ ﺳﻴﻨﺎ زاده 7 ) B.Sc. ( 1 - ﺳﻤﻨﺎن ﭘﺰﺷﻜﻲ ﻋﻠﻮم داﻧﺸﮕﺎه- داﻣﻐﺎن ﺷﻬﺮﺳﺘﺎن ﺑﻬﺪاﺷﺖ ﻣﺮﻛﺰ- ﺣﺸﺮه ارﺷﺪ ﻛﺎرﺷﻨﺎس و ﭘﺰﺷﻜﻲ ﺷﻨﺎﺳﻲ ﻧﺎﻗﻠﻴﻦ ﺑﺎ ﻣﺒﺎرزه. 2 - ﺗﻬﺮان ﭘﺰﺷﻜﻲ ﻋﻠﻮم داﻧﺸﮕﺎه- ﺣﺸﺮه ﮔﺮوه و ﭘﺰﺷﻜﻲ ﺷﻨﺎﺳﻲ ﻧﺎﻗﻠﻴﻦ ﺑﺎ ﻣﺒﺎرزه- اﺳﺘﺎد. 3 - داﻧﺸﮕﺎه ﺗﻬﺮان ﭘﺰﺷﻜﻲ ﻋﻠﻮم- ﺣﺸﺮه ﮔﺮوه و ﭘﺰﺷﻜﻲ ﺷﻨﺎﺳﻲ ﻧﺎﻗﻠﻴﻦ ﺑﺎ ﻣﺒﺎرزه- داﻧﺸﻴﺎر. 4 - ﺗﻬﺮان ﭘﺰﺷﻜﻲ ﻋﻠﻮم داﻧﺸﮕﺎه- اﻧﮕﻞ ﮔﺮوه ﻗﺎرچ و ﺷﻨﺎﺳﻲ ﭘﺰﺷﻜﻲ ﺷﻨﺎﺳﻲ- اﺳﺘﺎد. 5 - ﺗﻬﺮان ﭘﺰﺷﻜﻲ ﻋﻠﻮم داﻧﺸﮕﺎه- ﺣﺸﺮه ﮔﺮوه و ﭘﺰﺷﻜﻲ ﺷﻨﺎﺳﻲ ﻧﺎﻗﻠﻴﻦ ﺑﺎ ﻣﺒﺎرزه- ﻣﺮﺑﻲ. 6 - ﭘﺰﺷﻜﻲ ﻋﻠﻮم داﻧﺸﮕﺎه ﺳﻤﻨﺎن- داﻣﻐﺎن ﺷﻬﺮﺳﺘﺎن ﺑﻬﺪاﺷﺖ ﻣﺮﻛﺰ- ﻣﻴﻜﺮوﺑﻴﻮﻟﻮژي ﻛﺎرﺷﻨﺎس. 7 - ﺑﻬﺪاﺷﺖ وزارت، ﭘﺰﺷﻜﻲ آﻣﻮزش و درﻣﺎن- ﻛ ﺣﻮادث و ﺳﻮاﻧﺢ اداره ﺎرﺷﻨﺎس. درﻳﺎﻓﺖ ﺗﺎرﻳﺦ: 12 / 10 / 1388 ، ﭘﺬﻳﺮش ﺗﺎرﻳﺦ: 12 / 5 / 1389 ﭼﻜﻴﺪه ﻣﻘﺪﻣﻪ: ﻛﻪ ﺷﺪه ﺟﺪا ﺟﻮﻧﺪﮔﺎن و ﭘﺎﭘﺎﺗﺎﺳﻲ ﻓﻠﺒﻮﺗﻮﻣﻮس از ﻧﻴﺰ ﺗﻮراﻧﻴﻜﺎ ﻟﻴﺸﻤﺎﻧﻴﺎ اﻧﮕﻞ ﺑﻴﻤﺎري ﻧﻴﺴﺖ ﻣﺸﺨﺺ اﻧﺴﺎن در آن زاﻳﻲ. و ﻣﻮاد روش ﻫﺎ: ﺑﻪ ﻣﻄﺎﻟﻌﻪ اﻳﻦ ﺧﺎﻛﻲ ﭘﺸﻪ آﻟﻮدﮔﻲ ﺗﻌﻴﻴﻦ ﻣﻨﻈﻮر اﻧﮕﻞ ﺑﻪ ﻫﺎ اﺳﺖ ﺷﺪه اﻧﺠﺎم داﻣﻐﺎن ﺷﻬﺮﺳﺘﺎن روﺳﺘﺎﻳﻲ ﻣﻨﺎﻃﻖ در ﺗﻮراﻧﻴﻜﺎ ﻟﻴﺸﻤﺎﻧﻴﺎ. ﻣﻄﺎﻟﻌﻪ اﻳﻦ در ﺧﺎﻛﻲ ﭘﺸﻪ ﺑﻪ ﻫﺎ- ﺗﻠﻪ وﺳﻴﻠﻪ ﺷﺪه ﺻﻴﺪ ﭼﺴﺒﺎن ﻫﺎي اﻧﺪ. ﺧﺎﻛﻲ ﭘﺸﻪ اﺳﺘﺨﺮاج ﻓﺮاﻳﻨﺪ در ﺷﻨﺎﺳﺎﻳﻲ از ﭘﺲ ﻣﺎده ﻫﺎيDNA ﺗﻜﻨﻴﻚ در ﻟﻴﺸﻤﺎﻧﻴﺎ اﻧﮕﻞ ﺑﺮاي ﺟﺴﺘﺠﻮ وConventional PCR از اﺳـﺘﻔﺎده ﺑﺎ ﭘﺮاﻳﻤﺮﻫﺎيITS1 ) LITSR وL5.8S ( ﮔﺮﻓﺘﻨﺪ ﻗﺮار. ﻧﺮم ﺗﻮﺳﻂ ﺗﻮاﻟﻲ ﺗﻌﻴﻴﻦ واﻛﻨﺶ ﻣﺤﺼﻮﻻت اﻓﺰارBlast ﺷﺪﻧﺪ ﺗﺤﻠﻴﻞ و ﺗﺠﺰﻳﻪ. ﻧﺘﺎﻳﺞ: 218 ﭘﺎﭘ ﻓﻠﺒﻮﺗﻮﻣﻮس ﺧﺎﻛﻲ ﭘﺸﻪ زﻧﺠﻴﺮه واﻛﻨﺶ آزﻣﺎﻳﺶ ﻣﻮرد ﺎﺗﺎﺳﻲ ﮔﺮﻓﺘﻨﺪ ﻗﺮار ﭘﻠﻴﻤﺮازي اي. ﻣﻮرد ﻳﻚ اﻧﮕﻞ از اﺳـﺖ ﺑـﻮده ﺗﻮراﻧﻴﻜـﺎ ﻟﻴﺸـﻤﺎﻧﻴﺎ ﮔﻮﻧـﻪ ﺑﻪ ﻣﺮﺑﻮط ﺟﺪاﺷﺪه ﻫﺎي ) 5 / 0 .(% اﺳﺖ ﺷﺪه ﺛﺒﺖ اﻳﺮان در ﻣﺮﺗﺒﻪ دوﻣﻴﻦ و ﺳﻤﻨﺎن اﺳﺘﺎن در ﺑﺎر ﻧﺨﺴﺘﻴﻦ ﻳﺎﻓﺘﻪ اﻳﻦ. ﻧﺘﻴﺠﻪ ﮔﻴﺮي: ﺑﻴﻤﺎري اﻧﺴﺎن در ﺗﻮراﻧﻴﻜﺎ ﻟﻴﺸﻤﺎﻧﻴﺎ زاﻳﻲ ﻣﻮش در اﻣﺎ ﻧﻴﺴﺖ ﻣﺸﺨﺺ ﻣﻲ ﺑﻴﻤﺎري ﺑﺎﻋﺚ آزﻣﺎﻳﺸﮕﺎﻫﻲ ﻫﺎي ﺷﻮد. ﻣﻲ ﻧﺸﺎن ﻣﻄﺎﻟﻌﺎت دﻫ ﻨ در ﺪ ﺑـﻪ ﺟﻮﻧـﺪﮔﺎن ﻫﻤﺰﻣﺎن آﻟﻮدﮔﻲ اﻧﮕﻞ ﻣﺎژور و ﺗﻮراﻧﻴﻜﺎ ﻟﻴﺸﻤﺎﻧﻴﺎ ﻫﺎي، ﺑﻴﻤﺎري ﻗﺪرت ﻣﻲ اﻓﺰاﻳﺶ ﻣﺎژور ﻟﻴﺸﻤﺎﻧﻴﺎ زاﻳﻲ ﻳﺎﺑﺪ. ﻣﻲ ﻧﺸﺎن اﻳﻦ آﻧـ ﻣﻨـﺎﻃﻖ در ﺟﻠـﺪي ﻟﻴﺸﻤﺎﻧﻴﻮز ﺑﻪ اﻓﺮاد ﺑﺮﺧﻲ اﻳﻤﻨﻲ ﺷﺎﻳﺪ دﻫﺪ ﺑـﻪ ﺪﻣﻴﻚ ﻋﻠـﺖ ﻣﻲ ﮔﻮﻧﻪ اﻳﻦ و ﺑﺎﺷﺪ ﺗﻮراﻧﻴﻜﺎ ﻟﻴﺸﻤﺎﻧﻴﺎ ﺑﺎ ﻣﻮاﺟﻬﻪ ﺑﻪ ﺗﻮاﻧﺪ ﻛﺎﻧﺪﻳﺪي ﻋﻨﻮان ﮔﻴﺮد ﻗﺮار ﻣﺪﻧﻈﺮ واﻛﺴﻦ ﺳﺎﺧﺖ ﺟﻬﺖ. واژه ﻛﻠﻴﺪي ﻫﺎي: ﭘﺎﭘﺎﺗﺎﺳﻲ ﻓﻠﺒﻮﺗﻮﻣﻮس ﺗﻮراﻧﻴﻜﺎ، ﻟﻴﺸﻤﺎﻧﻴﺎ، ITS1 ، داﻣﻐﺎن. Original Article Knowledge & Health 2010;5(2,3):48-51 Isolation of Leishmania Turanica from Phlebotomus Papatasi Using ITS1 Sequencing at Zoonotic Cutaneous Leishmanianisis Focus in Damghan, Semnan Province Sadegh Mohammadi-Azni 1 ,Yavar Rassi 2* , Mohammad-Ali Oshaghi 2 , Mohammad-Reza Yaghoobi Ershadi 3 , Mehdi Mohebali 2 , Mohammad-Reza Abai 2 , Fatemeh Mohtarami 2 , Zeinab Nokandeh 1 , Sina Rafie-Zadeh 4 1- Damghan Health Center, Semnan University of Medical Sciences, Damghan, Iran. 2- Dept. of Medical Entomology and Vector Control, Tehran University of Medical Sciences, Tehran, Iran. 3- Dept. of Medical Parasitology, Tehran University of Medical Sciences, Tehran, Iran. 4- Ministry of Health and Medical Education, Tehran, Iran. Abstract: Introduction: L.turanica is isolated from Phlebotomus Papatasi and rodents, and its pathogenetic role in humans is not clear yet. Methods: This study aimed at determining the infection of sand flies to L.turanica in rural parts of Damghan. Sand flies were collected by sticky papers. After the identification of female specimens, their DNA was extracted and Conventional PCR technique using specific primers of ITS1 was used to search for L.turanica. Products of PCR were sequenced and analyzed with Blast software. Results: Two hundred and eighteen specimens of female P.papatasi were examined for Leishmania infection. One out of 218 females (0.5%) was positive to L.turanica. This is the first report of natural infection of P.papatasi due L.turanica in Semnan Province and the second in Iran. Conclusion: Pathogenesis of L.turanica is not clear on human, but, this parasite is pathogenic in laboratory mice. Furthermore, mix synchronized infection of rodents to L.major and L.turanica aggravates pathogenetic effect of L.major. Immunity of some people to Cutaneous Leishmanianisis in endemic areas can be due to the exposure to L.turanica and this can be a suitable candidate for vaccine investigations against cutaneous leishmaniasis. Keywords: Phlebotomus papatasi, Leishmania turanica, ITS1, Damghan. Received: 2 January 2010 Accepted: 3 August 2010 *Corresponding author: Y. Rasi, Email: rassiy@sina.tums.ac.ir