International Journal of Medicine and Medical Sciences, ISSN:2051-5731, Vol.46, Issue.4 1384 © RECENT SCIENCE PUBLICATIONS ARCHIVES| November 2013|$25.00 | 27702894 | *This article is authorized for use only by Recent Science Journal Authors, Subscribers and Partnering Institutions* Sub-Acute Toxicity Study of Puffer Fish Toxin (PFT), in Swiss Albino Mice, Extracted and Purified from Puffer Fish Tetraodon Cutcutia Collected from Coastline of Bangladesh M Moyen Uddin Pk Department of Biochemistry & Molecular Biology, Rajshahi University, Bangladesh Email: moyen.uddinpk@primeasia.edu.bd Rumana Pervin Department of Biochemistry & Molecular Biology, Rajshahi University, Bangladesh Email: rumana_pervin@yahoo.com Shahangir Biswas Department of Biochemistry & Molecular Biology, Rajshahi University, Bangladesh Email: bioshahangir@gmail.com Matiar Rahman Department of Biochemistry & Molecular Biology, Rajshahi University, Bangladesh Email: matiar_rahman@yahoo.com D Anwarul Azim Department of Biochemistry, Primeasia University, Dhaka-1213, Bangladesh Email: dewanazim@yahoo.com M Rubel Mia Department of Biochemistry, Primeasia University, Dhaka-1213, Bangladesh Email: rubelmia88@gmail.com Yearul Kabir Department of Biochemistry & Molecular Biology, University of Dhaka, Bangladesh Email: ykabir@yahoo.com Nurul Absar Department of Biochemistry & Molecular Biology, Rajshahi University, Bangladesh Email: nurul_ustc@yahoo.com ABSTRACT Puffer fish toxin (PFT), also called tetrodotoxin (TTX) found in puffer fish and little have known as tetrodotoxification in Bangladesh. In this study, our aimed to investigate the sub-acute toxicity of purified puffer fish toxin (PFT) from puffer fish liver to the Swiss albino mice (SAM). Preliminary toxicity of the different fish tissues namely liver, kidney, heart, and muscle of puffer fish, tetraodon cutcutia were assessed with brine shrimp lethality and bioassay resulting liver tissue was most toxic part of tetraodon cutcutia. The LC 50 of liver, kidney, muscle, heart, and skin tissue extracts were 0.97, 0.90, 1.30, and 1.45 μg/ml. And sub-acute toxicity of purified toxin, PFT from fish liver was confirmed by administrating of four different dose forms in SAM. Five groups of SAM consisting of each group with six male SAM were used. The group-I (G_I) served as a control and received a daily intraperitoneal injection of normal saline solution (1 ml / 100g of 0.9 % NaCl, V/W) , and group-II (G_II), group-III (G_III), group-IV (G_IV), and group-V (G_V) were received intraperitoneal injection of PFT/daily. During experiment, we observed a significantly decreased body weight & diet consumption rate in all PFT treated SAM but their increased the blood serum level of glutamic oxaloacetic transaminase (SGOT), glutamic pyruvic transaminase activities (SGPT), alkaline phosphatase (SAP), total protein (TP), total bilirubin, blood urea nitrogen (BUN) , creatinine, potassium , phosphorus, red blood cells, red cell indices, and leukocytes, while decreased in serum albumin, and serum calcium were ascribed with hepatoxicity, nephrotoxicity, hematoxicity , and cardiomyopathy in PFT treated SAM. In histotoxicity study, puffer fish toxicity observation revealed cellular toxicity especially in the liver kidney, lungs, and heart of experimental SAM. This sub-acute toxicity of PFT clearly indicates the life threatening, if used unprocessed puffer fish as food. Keywords- Puffer fish, hepatoxicity, nephrotoxicity, hematoxicity, and Tetraodon cutcutia 1. INTRODUCTION Puffer fish toxification in the world, and even in Bangladesh is not a new one, every year especially during the rainy season people are killed when they consumed