~ 2611 ~ Journal of Pharmacognosy and Phytochemistry 2018; 7(1): 2611-2614 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2018; 7(1): 2611-2614 Received: 17-11-2017 Accepted: 18-12-2017 Iqra Nazir Department of Aquaculture, College of Fisheries, G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India RS Chauhan Department of Aquaculture, College of Fisheries, G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India Priyanka Arya Department of Aquaculture, College of Fisheries, G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India Correspondence RS Chauhan Department of Aquaculture, College of Fisheries, G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India Evaluation of immunostimulatory effect of feed probiotic biosyn on Labeo rohita Iqra Nazir, RS Chauhan and Priyanka Arya Abstract The present study was carried out from February to May 2015 to evaluate the effects of dietary supplementation of commercial probiotic Biosyn in the fingerlings of Indian major carp (Labeo rohita) on haematological and biochemical parameters. Therefore, the fish with similar body weight (7.239 ± 0.232 g) were distributed randomly into four treatment groups T0, T1, T2 and T3. Experimental diets were prepared by mixing rice bran, deoiled mustard oil cake, soybean cake and vitamin mineral mixture. The biomedicine Biosyn was incorporated in to diet D1 @ 0.2%, D2 @ 0.4%, D3 @ 0.6%. In control diet D0, Biosyn was not incorporated in feed. The fishes were fed @ 5% body weight per day for 90 days. T3 group of fishes showed higher value of TLC (35.114×10 3 /μl), TEC (3.35×10 6 /μl), Hb concentration (10.2 g%), Total serum proteins (9.1g/dl), Albumin(1.695 g/dl) and Globulin (7.405 g/dl) as compared to control and other treatments. The obtained results indicated that T3 was the best treatment which realized significant (P<0.05) increase in all haematological and biochemical parameters. There were no adverse effects on water quality parameters among all the experimental treatments. Consequently, from the obtained results, it can be concluded that the inclusion of the commercial probiotic Biosyn @ 0.6% in diet of Labeo rohita is useful to get the best fish performance with friendly effects on the environment. Keywords: biomedicine, haematology, probiotic, total serum protein Introduction The demand for animal protein in India is expected to increase progressively with the increase in human population. Aquaculture is one of the most important options in animal protein production and requires high quality feeds with high protein content as well as some complementary additives to keep organisms healthy and favour the growth. The use of probiotics as farm animal feed supplements dates back to the 1970. One of the best methods to reduce feed cost is through the use of feed additives (Lara Flores et al., 2003) [12] . Their primary effects are to improve feed efficiency and / or daily gain. The use of these additives allows fish farmers to maximize the performance through improvement in health, reproduction and feed efficiency (Marzouk et al., 2008) [13] . Probiotics are live microorganism which can affect the host animal by improving their intestinal flora as supplements to improve growth performance (Kesarcodi Watson et al 2008) [11] and they are used as antimicrobial agents (Moriarty 1997) [15] . On the other hand, they are effective agents to decrease the effects of stress and resulted in higher production in aquaculture (Ghazalah et al 2010) [9] . Mechanism of action of probiotics is very different. Some of these mechanisms are included as inhibition of pathogens via production of antagonistic compounds, competition for attachment to cell, competition for absorption of nutrients, alteration of enzymatic activity of pathogens (Bomba et al 2002) [4] . Many studies showed the positive effects of viable microorganisms as probiotic in diets of fish (Brunt & Austin 2005; Pangrahi et al 2005; Barnes et al 2006; Abo-State et al 2009) [6, 16, 3, 1] . Probiotics must be considered as potentially useful for the control of fish diseases (Irianto & Austin 2002) [10] . In aquaculture, persistent disease problems necessitate the use of bacteria as probiotics and as alternatives to antibiotic. The common probiotics used in aquaculture, belonging to Lactobacillus sp., Bacillus sp., Bifidobacterium sp., Vibrio sp., Saccharomyces sp., Enterococcus sp. Bacillus subtilis, are now being used for oral bacteriotherapy in aquaculture. Biosyn is an optimum blend of feed probiotics and it consists of optimum number of colony forming units, counts of selected strains of bacteria which are capable of creating a healthy gut flora in fish. Probiotics in Biosyn helps in decomposition of excess and waste organic matter and suppress the growth of undesirable microbes in the pond and there by helps to keep the water clean. The present study was carried out to evaluate the effect of commercial probiotic Biosyn on haematological and biochemical parameters in Labeo rohita.