Int.J.Curr.Microbiol.App.Sci (2017) 6(7): 4595-4599 4595 Original Research Article https://doi.org/10.20546/ijcmas.2017.607.481 Therapeutic Efficacies of Self-Formulated Anti-Oxidant Trace Minerals Formulation in Bovine Mastitis A. Muhee 1* , H. U. Malik 1 , I. Asharaf 1 , O. S. Shah 1 , A. Jan 1 , Muheet 1 , W. Rather 1 and Showkeen Muzamil 2 1 Department of Veterinary Medicine ethics and jurisprudence, F.V.Sc & A.H, SKUAST-K, Srinagar- 192301, Jammu and Kashmir, India 2 Molecular Biology Lab, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences & Animal Husbandry, Sheri Kashmir University of Agricultural Science & Technology-Kashmir (SKUAST-K), Srinagar, J&K-190006, India *Corresponding author ABSTRACT Introduction Mastitis is a parenchymal inflammation of the mammary gland, which is caused by microorganisms, usually bacteria that invade the udder, multiply and produce toxins which are harmful to the mammary gland (Sharma et al., 2006). Mastitis could induce increased formation of free radicals in milk leading to oxidative stress (Gu et al., 2009). Substantial amounts of free radicals are produced during an inflammatory response such as that which occurs when the mammary gland becomes infected. Antioxidant supplementation could decrease the duration; incidence and severity of clinical mastitis as the formation of increased free radicals during mastitis are mostly associated with decreased antioxidant defence. Trace elements with anti-oxidant function include copper, zinc, manganese, iron and selenium which act as co-factors for anti-oxidant enzymes superoxide dismutase, catalase and glutathione peroxidase which are directly or indirectly involved in quenching or The study was undertaken on clinical cases of bovine mastitis presented to Teaching Veterinary Clinical Complex (TVCC), F.V.Sc & A.H, SKUAST-K and adjoining areas during the course of study August 2014-May 2016. For therapeutic trials, the clinical cases of mastitis were divided into two groups. In Animals of Group I therapeutic regimen I was used conducted and included 15 clinical cases of mastitis, in Group II animal’s therapeutic regimen II was used and included 12 clinical cases of mastitis. The animals of group II were given self-formulated anti-oxidant trace mineral mixture. Group II animals were not given any anti-oxidant trace mineral mixture. The requirements of trace minerals like Cu, Zn, Mn and Se are increased in mastitis as evidenced by their decreased levels in clinical mastitis and improved clinical response on their supplementation. Anti-oxidant trace minerals like Cu, Zn, Mn and Se significantly reduce oxidative stress and augment clinical recovery and may play a significant role in treatment of mastitis in lactating animals. Keywords Therapeutic efficacies, self-formulated anti- oxidant Accepted: 28 June 2017 Available Online: 10 July 2017 Article Info International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 7 (2017) pp. 4595-4599 Journal homepage: http://www.ijcmas.com