www.ijbcp.com International Journal of Basic & Clinical Pharmacology | September 2019 | Vol 8 | Issue 9 Page 2134 IJBCP International Journal of Basic & Clinical Pharmacology Print ISSN: 2319-2003 | Online ISSN: 2279-0780 Original Research Article Evaluation of dose dependent analgesic response by extracts of Myristica fragrans on albino wistar rats: an experimental study Imran Zaheer 1 *, Syed Ziaur Rahman 2 , Rahat Ali Khan 2 , Mehtab Parveen 3 , Priyamvada Sharma 1 INTRODUCTION Pain is a common subjective phenomenon which brings a patient to physician. It is associated with a number of diseases and is estimated that 80–100% of the population experience back pain at least once in the life time. 1 Non- steroidal anti-inflammatory drugs (NSAIDs) are the main stay of treatment of pain. 2 It is known fact that the risk of gastrointestinal bleeding and other side effects are associated with acute and chronic use of non-steroidal anti-inflammatory drugs (NSAIDs). 3 Keeping in view the gravity of adverse effects of NSAIDs it is necessary to search for new drugs with less adverse effects. In line with this many traditional medicinal plants have been used time to time for the development of new drugs with comparatively less ADRs as compare to NSAIDs. 4 Recently, many natural medicines derived from medicinal plants, were considered as the effective and safer for the treatment of various diseases including inflammation and pain. 5 One such medicinal plant namely Myristica fragrans is claimed for it is valuable role in reducing pain and inflammation. Hence, it was ABSTRACT Background: The objective of the study was to evaluate analgesic activity of ethanolic extract, methanol and benzene fraction of Myristica fragrans on wistar albino rats. Methods: The present study was carried out in the department of pharmacology JNMC AMU and F.H. Medical College, Agra. The analgesic activity was evaluated by employing the Eddy’s hot plate method and tail flick response method. In both the tests, Rats of either sex weighing 150-200 g were used. The total number of animals n=36 were allocated to six groups. Each group consist of six animals each. The response noted in animals that were tested by hot plate method was reaction time for licking/biting of both the paws before and after administration of control & test drugs. However in Tail flick test, the pain threshold response was recorded before and after administration of control & test drugs. The statistical analysis was done by using one-way ANOVA. The data is expressed as Mean±SEM. P<0.05 was considered to be statistically significant. Results: Ethanolic extracts and methanol fraction of M. fragrans showed statistically significant (p<0.001) increase in reaction time for licking/biting in hot plate method. On the contrary a significant increase in pain threshold was also recorded in tail flick response test. It is interesting to note that no significant degree of analgesia related to any dose of benzene fraction was observed. Conclusions: The present study reveals the dose dependent significant analgesic activity of the extracts of M. fragrans i.e. ethanolic extracts and methanol fraction in both the test. However, the degree of analgesia was recorded significantly higher in groups received higher doses of extracts of M. fragrans. Keywords: Analgesic activity, Myristica fragrans, Albino wistar rats DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20194127 Department of Pharmacology, 1 F.H. Medical College and Hospital, Etmadpur, Agra, 2 Jawaharlal Nehru Medical College, 3 Department of Chemistry F/O Science, Aligarh Muslim University, Aligarh, Uttar Pradesh, India Received: 13 July 2019 Accepted: 16 August 2019 *Correspondence to: Dr. Imran Zaheer, Email: imran82zaheer @gmail.com Copyright: © the author(s), publisher and licensee Medip Academy. This is an open- access article distributed under the terms of the Creative Commons Attribution Non- Commercial License, which permits unrestricted non- commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.