Available online freely at www.isisn.org Bioscience Research Print ISSN: 1811-9506 Online ISSN: 2218-3973 Journal by Innovative Scientific Information & Services Network RESEARCH ARTICLE BIOSCIENCE RESEARCH ,2018 15(3):1695-1702 OPEN ACCESS Selection of deleterious rhizobacterial isolate as bioherbicide to control of weed paspalum conjugatum and ageratum conyzoides on soybean cropland Tresjia Corina Rakian 1* Muhidin 1 , GustiAyuKade Sutariati 1* , Gusnawaty HS 2 , Asniah 2 and Uli Fermin 1 1 Department of Agrotechnology, Faculty of Agriculture, Halu Oleo University, Kendari Southeast Sulawesi, Indonesia 2 Department of Plant Protection, Faculty of Agriculture, Halu Oleo University, Kendari 93232, Southeast Sulawesi, Indonesia *Correspondence: tresjia_junus@yahoo.com Accepted: 09 jun.2018 Published online: 04 Aug. 2018 Rhizobacteria has a potential to control weed that can act as a bioherbicide. Rhizobacteria that has ability to inhibit growth of weed called as deleterious rhizobacteria (DRB). The study aimed to get indigenous rhizobacterial isolates from weed rhizosphere of soybean that has potential as bioherbicide and can control the weed growth. The research was conducted in Agronomy Laboratory, Faculty of Agriculture, Halu Oleo University. The research consisted of four steps as follows: (1) Isolation of rhizobacterial from weed rhizosphere of soybean, (2) Evaluation of rhizobacterial ability as bioherbicide, (3) Rhizobacterial treatment on weed seed and (4) Observation of weed seed germination. Data were analyzed using two-ways analysis of variances (ANOVA) and Statistical Package of Social Sciences (SPSS). The research found 45 indigenous rhizobacterial isolates, based on the differential morphological character from nine kinds of dominant weeds in soybean cropland. The results showed that some indigenous rhizobacterial isolates has potential as bioherbicide and suppress the viability and seed vigor of Paspalumconjugatum and Ageratum conyzoides. The most effective indigenous rhizobacterial isolate to a control of weed is Pk02 isolated from the rhizosphere of P. conjugatum and A08 isolated from the rhizosphere of A. conyzoides. Keywords: Bioherbicide, weed control, Paspalumconjugatum, Ageratum conyzoides, deleterious rhizobacteria, rhizosphere INTRODUCTION Soybean (Glycine max. (L.) Merrill) a leguminous plant is one of the functional food and important agricultural commodities around the world (Medi et al., 2014) including Indonesia. Soybean contains amounts of nutrients such as proteins (Celemente et al., 2009; Lamb, 2011; Krishnan and Darly-Kindelspire, 2013; Tripathi et al., 2015), fatty acids, minerals, vitamins and constitutes a well-known source of bioactive phytochemicals (Weber et al., 2005; O’Bryan et al., 2014) with health-promoting effects Bora, 2014; Kanchana et al., 2016). Soybean are also ecofriendly, cheap source of food (Odiase et al., 2013; Ciabotti et al., 2016) and beneficial for health (Wang et al., 2004; Ferreira et al., 2011; Sharma and Baluja, 2015) in comparison with conventional legume and animal food sources. Soybean in Indonesia serves as a major human food due to its nutritional and health