5 The Journal of Phytopharmacology 2020; 9(1): 5-11 Online at: www.phytopharmajournal.com Research Article ISSN 2320-480X JPHYTO 2020; 9(1): 5-11 January- February Received: 10-01-2020 Accepted: 08-02-2020 ©2020, All rights reserved doi: 10.31254/phyto.2020.9102 Adédoyin A Rafatou Adjileye Laboratory of Biochemistry and Bioactives Natural Products, Unit of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of Abomey-Calavi, Benin Abdou Madjid O Amoussa Laboratory of Biochemistry and Bioactives Natural Products, Unit of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of Abomey-Calavi, Benin Rafiou Adamou Laboratory of Biochemistry and Bioactives Natural Products, Unit of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of Abomey-Calavi, Benin Bonaventure Awede Unit of Physiology, Faculty of Health Sciences, University of Abomey-Calavi, Cotonou, Benin Ambaliou Sanni Laboratory of Biochemistry and Bioactives Natural Products, Unit of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of Abomey-Calavi, Benin Anatole Laleye Unit of Human Biology, Faculty of Health Sciences, University of Abomey- Calavi, Cotonou, Bénin Latifou Lagnika Laboratory of Biochemistry and Bioactives Natural Products, Unit of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of Abomey-Calavi, Benin Correspondence: Latifou Lagnika Laboratory of Biochemistry and Bioactives Natural Products, Unit of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of Abomey-Calavi, Benin Email: llagnika@gmail.com Antihypertensive effect of Dialium guineense Wild. and Trema orientalis L. in L-NAME-induced hypertensive rats Adédoyin A Rafatou Adjileye, Abdou Madjid O Amoussa, Rafiou Adamou, Bonaventure Awede, Ambaliou Sanni, Anatole Laleye, Latifou Lagnika* ABSTRACT Dialium guineense (Caesalpiniaceae) and Trema orientalis (Celtidaceae) are traditionally used to treat and manage many diseases such as hypertension, headache and diabetes. Despite their recognized efficacy in traditional medicine, their antihypertensive properties are not yet effective. Therefore, the current study aimed to investigate the antihypertensive effect of ethanolic extracts of Dialium guineense and Trema orientalis on L-NAME-induced hypertensive rats. The systolic, diastolic and mean arterial pressure were recorded using CODA™ non-invasive blood pressure system. The phenolic compounds were also quantified using High Pressure Liquid Chromatography. Ethanolic extracts of both plants induced significant decrease of mean arterial pressure. At 500 mg/kg bw, both plants decreased mean arterial pressure from 126.4 ± 0.48 to 90.6 ± 3.12 mmHg and from 154.8 ± 7.84 to 103 ± 5.6 mmHg respectively for Dialium guineense and Trema orientalis. Losartan and Captopril, used as standard drugs at 100 mg/kg body weight, also significantly decrease blood pressure. Trema orientalis was the most active with a blood pressure reduction percentage of 33.46 ± 3.06 % comparable to Losartan (31.37 ± 3.13 %) and Captopril (31.76 ± 2.63 %). The probable bioctives compound identified were chlorogenic, gallic, caffeic, ellargic, tannic acids, and luteolin, isorhamnetin and chrysin. Keywords: Dialium guineense, Trema orientalis, Antihypertensive activity. INTRODUCTION All over the world, human health is affected by the same problems, such as the aging of the population, rapid urbanization and the spread of unhealthy lifestyles. Poeple in developed and low income countries face the same health problems, among which the most important is the increase of cases of non transmissible diseases such as cardiovascular diseases, cancer, diabetes and chronic lung diseases [1] . Although World Health Organization announced that governments ensure that all people have equitable access to the preventive, curative and rehabilitative health services they need to avoid developing high blood pressure and its complications, it is clear that the road ahead to achieve this goal is still long [1] . In spite of the increase of accessibility of modern medicine and the variety of drugs available for various ailments, it has been observed that 85% of patients combine herbal therapy with the medicines prescribed at hospitals or clinics [2] . The plant-derived remedies or recipes are widely used in all civilizations and cultures. Hence, plants have always played an important role in health care systems worldwide [3] . Despite the availability of various approaches and strategy for the discovery of new drugs, natural products still remain as one of the best reservoirs of new structural types of compounds. Indeed, It is well known that plants are a valuable source of new natural and pharmaceutical products. It has also been reported that plants and plant-based products constitute the bases of many modern pharmaceuticals used today for the treatment of various ailments [4] . In Bénin, medicinal plants are used in the managment of various diseases including hypertension (HTA) [5, 6] . These plants include Dialium guineense Wild., and Trema orientalis L. belonging to the family Caesalpiniaceae and Celtidaceae respectively [7] . Dialium guineense is widely used in folk medicine to manage various ailments [8, 9] whereas Trema orientalis has been reported for the management of some diseases such as hypertension, malaria and diarrhea [10, 11] . Several biological properties such as antiplasmodial, antioxidant, analgesic, anti-microbial were also carried out on Trema orientalis [12, 13] . Despite their frequent use and studies, as far as we know, there is no report on the antihypertensive activity of Dialium guineense and little data are available about the antihypertensive activity of Trema orientalis. Consequently, the effect of chronic administration of ethanolic extracts of Dialium guineense and Trema orientalis on L-NAME-Induced Hypertensive Wistar rats was investigated and the probable bioactives phenolic secondary metabolites were quantified.