Abstract—Study is focused on the concentration of selected elements that affect the living organism as well as individual organs. Samples of uterus and ovaries of dogs and cats were analyzed using absorption spectrometry. The average, minimum and maximum concentrations in the samples were detected and subsequently the correlations between elements were calculated. The average concentration of sodium, potassium, iron and zinc were higher in cats. Higher concentration of calcium and copper were found in the bitch samples. The cadmium concentration in cats was slightly higher compared to bitch. Lead concentrations in the samples of studied cats were 0.28±0.16 mg/kg and in female dogs were 0.24±0.14 mg/kg, mercury concentration reached the 0.01±0.01 mg/kg for cats and 0.00±0.00 mg/kg for dogs. In cats, a strong negative correlation between sodium and calcium, and a strong positive correlation between the potassium and the calcium and copper and zinc were found. Correlation analysis in female dogs showed strong correlation between sodium and potassium, sodium and copper, potassium and zinc, potassium and cadmium, potassium and lead, iron and mercury, calcium and copper, calcium and mercury, copper and zinc, copper and cadmium as well as the lead and copper and a strong positive correlation between sodium and zinc, sodium and cadmium, sodium and lead, iron and calcium, zinc and cadmium, zinc and lead, and the lead and cadmium. Pets can serve as indicators of environmental metal pollution monitored since as they inhabit the same space as men and are exposed to the same contaminants. Correlations observed for analyzed elements indicate interrelationships of monitored elements in the animal reproductive organs. Index Terms—Sodium, potassium, iron, calcium, copper, zinc, cadmium, lead, mercury, reproductive organs, cat, dog. I. INTRODUCTION The unrestricted developmental activities such as industrialization and urbanization carried out during the past few decades have given rise to serious problems of environmental pollution [1]. Contamination of the food chain with heavy metals may negatively influence both the health status as well as animal production. Unfavorable effect on the animal health may depend on the kind of the element and its dose as well as on the utility orientation [2]. Lead (Pb) and cadmium (Cd) are important environmental Manuscript received June 28, 2016; revised September 6, 2016. This work was supported by projects VEGA 1/0760/15, 1/0857/14, APVV-0304-12 and KEGA 006/SPU-4/2015. P. Massanyi, N. Knizatova, and M. Massanyi are with the Slovak University of Agriculture in Nitra, Department of Animal Physiology, Slovak Republic (e-mail: massanyip@gmail.com). L. Pavlik is with Private Veterinary Service in Nitra, Slovak Republic and Slovak University of Agriculture in Nitra, Department of Animal Physiology, Slovak Republic. R. Stawarz and P. Massanyi are with Pedagogical University of Krakow, Institute of Biology, Poland. pollutants present in soil, water and air. Their common sources are diverse including natural and anthropogenic processes such as combustion of coal and mineral oil, smelters, mining, as well as paint industries [3]-[6]. Anthropogenic activities and vehicular emissions contribute to the entry of toxic metals to human and animal food chains [7]. Pb does not have any detectable beneficial biological role. On the contrary, its detrimental effects on physiological, biochemical, and behavioral dysfunctions in animals and humans have been documented by several investigators [8]-[10]. Higher levels affect the central and peripheral nervous system [11], haemopoietic system [12], cardiovascular system [13], kidneys and liver [10]. Pb and Cd contamination has also been associated with male reproductive toxicity in experimental animals and has the potential to produce adverse effects on fertility [14]. Pb and Cd-induced tissue damages have been attributed, at least in part, to toxicant-induced oxidative stress [5], [15]. The aim of this study was to determine the concentration of sodium, potassium, iron, calcium, copper, zinc, cadmium, lead and mercury in reproductive organs (ovaries, uterus and fallopian tube) in female cats and dogs and to investigate potential correlations between these elements. II. MATERIAL AND METHODS A. Material Samples were collected during castration and stored at -20°C during assembling. All animals came from Nitra region and at the time of sampling ranged in age from 8 months to 2 years. The majority of individuals were sexually mature and 2 females were pregnant. In the study 11 animals were analyzes, 8 cats and 3 female dogs. B. Methods For mercury analysis 200 μL of sample were used in the measurement. Mercury analyzer NIC MA-2 was used. Samples for mercury were not mineralized in the mineralizer (everything was done in the apparatus). Each sample was analyzed twice and if the RSD between replicates was lower than 10%, the mean was calculated and used as the final results. If RSD was higher, the sample was re-analyzed. Quality of the analysis was assessed with CRM and spikes analyses (all recoveries were between 90 and 110%). Limit of detection 0.1 ng; limit of quantification 0.208 ng [16]. For other metals 3 mL of sample were mineralized with the nitric acid (65%, Baker Analyzed) in the open mineralization system (VelpScientifica DK20). Mineralized samples were Biogenic and Risk Elements in Reproductive Organs of Female Cats and Dogs Peter Massanyi, Nikola Knizatova, Martin Massanyi, Lubomir Pavlik, and Robert Stawarz International Journal of Environmental Science and Development, Vol. 8, No. 2, February 2017 107 doi: 10.18178/ijesd.2017.8.2.930