Vol. 13 | No. 4 |2074-2084| October - December | 2020 ISSN: 0974-1496 | e-ISSN: 0976-0083 | CODEN: RJCABP http://www.rasayanjournal.com http://www.rasayanjournal.co.in Rasayan J. Chem., 13(4), 2074-2084(2020) http://dx.doi.org/10.31788/ RJC.2020.1345697 ISOLATION OF LIPOLYTIC BACTERIA FROM DOMESTIC WASTE COMPOST AND ITS APPLICATION TO BIODIESEL PRODUCTION Purkan Purkan 1,* , Indah Tri Lestari 1 , Rizky Arissirajudin 1 , Retno Rahayu Puspita Ningsih 1 , Wiwin Apriyani 1 , Hamidah Nurlaila 1 , Sri Sumarsih 1 , Sofijan Hadi 1 , Wiwin Retnowati 3 and Seung Wook Kim 1,2 1 Chemistry Department, Faculty of Science and Technology, Airlangga University. Jl. Mulyorejo, Surabaya 60115, Indonesia. 2 Department of Chemical and Biological Engineering, Korea University, Korea 3 Microbiology Division, Faculty of Medicine, Airlangga University, Jl Prof Dr. Moestajab, Surabaya, Indonesia *E-mail: purkan@fst.unair.ac.id ABSTRACT The exploration of lipolytic bacteria presents its challenges in being able to supply lipase as a catalyst in the production of biodiesel. The compost is chosen to get the bacteria because of the lipid and fatty acids component contained in it. The research was developed to isolate the lipase-producing bacteria and determine the enzyme ability in bioconversion of lipid to biodiesel. The bacterial screening was conducted in Luria Bertani media with additional olive oil 1% (v/v) as an inducer. The colonies surrounded by bright areas were separated from others because they indicated having lipolytic activity. Identification of selected isolate was carried out according to the 16srRNA fragment DNA analysis. The fragment showed a score of nucleotide identity as 98% with the Proteus sp. The isolated bacteria grow well in LB media forming an exponential phase from 2 to 11 h, and have a double-time of cell growth at the 4 h. It produced lipase optimally at the 19 hours with its activity of 1.771 U/mL. The enzyme showed optimum activity at 45 o C and pH 8. The lipase showed high activity in t-butanol then followed by sequentially in a mixed solvent (methanol and t- butanol), and a single solvent of isopropanol, methanol, and n-hexane. It exhibited good performance for the biodiesel production from microalgal oil, yielded a total fatty acid methyl esters (FAME) as 2.75% (v/v). The fatty acid composition of the methyl esters was composed of 9 octadecanoic acid and hexadecanoic acid. Keywords: Lipolytic Bacteria, Lipase, Biodiesel, 16srRNA. © RASĀYAN. All rights reserved INTRODUCTION Along with population growth, energy needs for fuel are estimated to continue to increase by 50 % or more by 2030 1 . In a developing country such as Indonesia, fuel is obtained by processing various kinds of fossil resources. The continual use of fossil fuel as a source of energy has an impact on the depletion of fossil fuel reserves and an increase in the amount of CO2 in the atmosphere. Today, research for environmentally friendly and renewable energy is an important challenge. The main alternative to replace fossil fuels that are currently being developed is biodiesel. Biodiesel is generally defined as methyl (ethyl) esters of fatty acids produced through transesterification (alcoholysis) of triglycerides 36 . Biodiesel is a product of alkyl ester fatty acids (C14-C22 long-chain) with a short chain of primary alcohols, methanol, or ethanol 2 . Biodiesel is a renewable energy source that is environmentally friendly because it can be easily degraded, has a low toxic effect, produces little waste and does not increase carbon dioxide, aromatic components, or other chemical substances that are harmful to the environment 3 . One of the factors that influence the efficiency of biodiesel production is the choice of the type of catalyst used in transesterification. One of the catalysts that can be used in biodiesel production is lipase. Lipase in biodiesel production has functions to convert triglycerides to methyl (ethyl) esters through a