8 th International Symposium on Tilapia in Aquaculture 2008 921 FATTY ACID COMPOSITION OF NILE TILAPIA OREOCHROMIS NILOTICUS MUSCLES: A COMPARATIVE STUDY WITH COMMERCIALLY IMPORTANT TROPICAL FRESHWATER FISH IN PHILIPPINES ASHRAF SULOMA 1 , HIROSHI Y. OGATA 2 , ESTEBAN S. GARIBAY 3 , DENNY R. CHAVEZ 3 AND EHAB R. EL-HAROUN 1, 4 1. Animal Production Department, Faculty of Agriculture, Cairo University (El Gamaa St. Giza, Egypt) 2. Fisheries Division Japan International Research Center for Agricultural Sciences (Tsukuba, Ibaraki, 305-8686 Japan). Present address: Southeast Asian Fisheries Development Center, Aquaculture Department (SEAFDEC/ AQD) (Tigbauan 5021, Iloilo, the Philippines) , t t t , 3. Aquaculture Departmen s, Southeast Asian Fisheries Development Cen er (Tigbauan 5021, Iloilo, the Philippines) 4. Center for Nutrition Modelling, Animal& Poultry Science Department, Universi y of Guelph, Guelph, Ontario, N1G2W1 Canada Abstract Six tropical freshwater species were collected from Philippines in order to study the characteristic of polyunsaturated fatty acids distributions. 16:0 and 18: l n-9 were the predominant saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) in both neutral lipids (NL) and polar lipids (PL). There was an absence or very low values of n3 highly unsaturated fatty acid (HUFA) in NL of all species. However these fatty acids found in PL of all species studied with higher levels. The high proportions of docosahexaenoic acid (DHA) in PL were found with catfish Arius (20.71%), ayungin (17.64%), and snakehead (17.09%) whereas anabas (4.21%) gave lower DHA content. In PL, arachidonic acids (ARA) was found in high proportions, and also is superior to eicosapentaenoic acid (EPA) (ranged from 3.35 to 10.67% and from 0.42 to 4.74%, respectively). Tilapia lipid appears to be intermediate in nutritional quality between all species studied; the proportions of DHA, EPA and ARA in PL were 16.27%, 4.52% and 9.36%, respectively. According to the n-3/n-6 ratio in both fractions, only ayungin is in the range typical of freshwater fish. Our results indicate that the wild tropical freshwater fish studied here are not good sources of n-3 HUFA fatty acids. Therefore, aquatic nutritionists and farmers should combine their efforts in order to manipulate the nutritional quality of these species to enhance their n-3 HUFAs concentrations especially when these species are reared in captivity system. The wash-out strategy may provide an adequate description of the changes in the fillet lipid fatty acid profiles of fatty fish. Keywords: Tropical freshwater fish; Polar lipids; Neutral lipids; docosahexaenoic acid; eicosapentaeoic acid; Arachidonic acid INTRODUCTION Most developing countries are located in tropical or sub-tropical areas, and fish is a vital component of food security for these countries. Rivers and lakes in these