J nat Rubb Res, 12(2), 120-130 Correlation between Total Extractable Proteins and Allergen Levels of Natural Rubber Latex Gloves ESAH YIP** T. PALOSUO**, H. ALENIUS** AND K. TURJANMAA*** Certain proteins or peptides eluting from natural rubber (NR) latex products can cause immediate hypersensitivity reactions (Type I allergy) in subjects sensitised to them The amount of total extractable proteins in manufactured latex products is believed to reflect reasonably well their corresponding allergenic protein level, but only a few studies have been published to substantiate this. The aim of the present study is to compare a widely used total protein measurement assay, namely, the RRIM modified Lowry test (EP RRIM ), to latex allergen analysis, carried out by specific IgE-ELISA-mhibition tests. A series of 46 widely marketed medical NR latex gloves was investigated. Their EP RR}M values ranged from < 20 fjg/gto 1290 jug/g, and their allergen content varied from < 1 AU/ml to 570 AU/ml In the measurement of allergen contents, the reference allergen mixture was prepared from serum proteins of fresh Hevea latex, and IgE antibodies were sourcedfrom both adults and spma bifida children sensitive to latex Results showed that the allergen levels were very well correlated with the total extractable protein contents (coefficient r = 0.89, P<0001, n = 46) With the exception of a few, gloves with high total extractable proteins were generally found to have high allergen contents, and vice versa. Gloves with EP^^ levels of 0.1 mg/g or 1QQ ng/g and below always had very low allergen contents (< 9 AU/ml). These findings are consistent with those shown by the in-vivo skin-prick test reported earlier. More importantly, they confirm the very low allergen levels observed at EP RR]M levels of about 100 ftg/g and lower Such information provides useful guideline for the manufacturing of reduced risk NR latex gloves. Type I allergy affecting certain individuals been placed on the production of latex devices through the use of some NR latex products, with better biocompatibihty. Many attempts has caused great concern among the latex have therefore been made to reduce their total product manufacturers. Emphasis has since extractable proteins shown to be implicated in *Rubber Research Institute of Malaysia, P O Box 10150, 50908 Kuala Lumpur ** National Public Health Institute, Helsinki, Finland Department of Dermatology, Tampere University Hospital, Tampere, Finland # Corresponding author 120