A New Microscopy Facility for Optical, Electron and X-Ray Imaging at Diamond Paul Quinn 1 , Julia Parker 1 and Fernando Cach-Nerin 1 , Dogan Ozakaya 2 , Judy Kim 1,3 and Angus Kirkland 1, 3 1. Diamond Light Source Ltd., Harwell Science and Innovation Campus, Didcot, UK. 2. Johnson Matthey Technology Centre, Sonning Common, Reading, Reading, UK 3. Department of Materials, Parks Road, Oxford, UK. Multi-scale, multi-technique experiments combining optical, X-ray and electron microscopy enable studies of systems and processes at different levels of complexity. This broader view is obtained from the differences in resolution and more importantly when the physical properties of the probes such as penetration depth or contrast mechanisms are exploited to view the sample or process in a different light. In the life sciences fluorescence microscopy is increasingly used to identify areas and events of interest within cells which are then examined at high resolution by electron microscopy. More recently these techniques have been combined with soft X-ray cryo transmission microscopy to bridge the length scales between these techniques to provide a more complex, complimentary view of a full (non- sectioned) cell [1]. In the physical sciences a recent study of nanocatalysts in operando conditions which correlated scanning transmission electron microscopy and X-ray spectroscopy clearly demonstrates the potential of an integrated approach [2]. The advantages of combining these various techniques are clear but they require substantial resources for equipment, infrastructure and expertise. To exploit the latest cutting edge developments in electron microscopy and to facilitate correlation of these techniques a new facility housing the I14 beamline and a National Electron Microscopy Facility for Physical Sciences (EMPSF) will open in late 2016 at Diamond Light Source. Within the EMPSF, Johnson Matthey, the University of Oxford and Diamond Light Source are collaborating on the creation of a state-of-the-art materials characterization facility. The core builiding will also house a National Facility for Cryo-Electron Microscopy - the electron Bio- Imaging Centre (eBIC) – with two advanced electron microscopes funded by The Wellcome Trust, MRC and BBSRC, in an initiative led by Birkbeck College, London and the University of Oxford [3]. The I14 beamline is a 185m long facility for hard X-ray scanning probe microscopy which will operate over a 4.5-26keV range and deliver an initial probe size of 30 nm. The experimental station is designed for scanning X-ray fluorescence, X-ray spectroscopy and diffraction and features a cryo-sample mount with rotation for cryo-tomography. eBIC will provide two state-of-the-art 300 kV FEI Titan-Krios cryo-microscopes with stable, cartridge- type sample holders and the latest generation of electron detectors, incorporating correction for beam- induced motion, as well as phase plates. The demand for these facilities is such that the first of these instruments was installed on the synchrotron hall floor in April 2015 and has been operating a full user program since startup. The second instrument is to be installed within the synchrotron in early 2016 with both devices subsequently migrating to the new building. Oxford University will bring a unique JEOL 300kV electron microscope dedicated to atomic scale imaging at world-leading resolution over a range of primary voltages and Johnson Matthey will install a 212 doi:10.1017/S1431927616001914 Microsc. Microanal. 22 (Suppl 3), 2016 © Microscopy Society of America 2016 https://doi.org/10.1017/S1431927616001914 Downloaded from https://www.cambridge.org/core. IP address: 54.70.40.11, on 17 Jan 2019 at 08:16:47, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms.