---------------------------------------------------------------------------------------------------------------------------------------- Archives of Hygiene SciencesVolume 5, Number 3, Summer 2016 © 2016 Publisher: Research Center for Environmental Pollutants, Qom University of Medical Sciences. All rights reserved. • Arch Hyg Sci 2016;5(3): 192-98 • Journal Homepage: http://jhygiene.muq.ac.ir RESEARCH ARTICLE 192 Roohollah Farhadloo a , Sarallah Shojaei b , Jalil Goodarzi Fard a , Mohammad Reza Azadeh a ,Mohammad Parvaresh Masoud c * Alireza Shouri Bidgoli d , Mostafa Vahedian e a Emergency Medical Center, Qom University of Medical Sciences, Qom, Iran. b Department of Health Education, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. c Department of Medical Emergency, School of Paramedical, Qom University of Medical Sciences, Qom, Iran. d School of Paramedical Sciences, Qom University of Medical Sciences, Qom, Iran. e Clinical Research Development Center, Qom University of Medical Sciences, Qom, Iran. *Correspondence should be addressed to Mr. Mohammad Parvaresh Masoud, Email: M_parvaresh_m@yahoo.com Presently, World Health Organization (WHO) and its affiliates are integral parts of a social institute which is responsible for provision of full health services for the public as a center for training of health staff and other segments of the society. Keeping people healthy, especially those individuals who deal with in hospitals and related centers, has a direct relationship with getting a proper insight into the factors giving rise to nosocomial infections (1). Since those centers are among important centers to deal with patients, so, nosocomial infections would be unavoidable in case of health and safety issues which are not taken into sufficient account. Having their roots in microorganisms A-R-T-I-C-L-EI-N-F-O A-B-S-T-R-A-C-T Article Notes: Received: Mar 4, 2016 Received in revised form: May 23, 2016 Accepted: June 25, 2016 Available Online: July 14, 2016 Background & Aims of the Study: When the issue of the quality of the patient's care is addressed, determination of infection degrees in reference to the quality of such cares has gained a high priority. Therefore, infections in ambulance equipment might play a significant role in reduction of the quality of the hospital cares. This study was conducted wishing to determine bacterial infection degrees in ambulances servicing in pre-hospital emergency medical services of Qom University of Medical Sciences, Iran. Materials and Methods: In this analytical cross-sectional study, 132 sampling of the equipment's of 12 ambulances were done. Samples were stored on BHI broth as an amplifier for 24 hours. Then, they were introduced to blood agar and Eosin Methylene Blue (EMB) agar culture environments. After 24 hours, negative staphylococcus coagulase and bacillus were specified by different environments, solutions, diagnostic discs, gram staining, catalase test, oxidase test and coagulase test. Then data were analyzed by SPSS16. Results: The results showed that the highest infection prevalence rate was observed in stretchers (12 samples, 100%) and the lowest in oxygenation moisturizers (1 sample, 8.4%). From infected equipment's, in addition, four instruments (37%) were mobile and eight of them (63%) were immobile. Conclusion: Results indicated that an infection degree of the utensils and instruments which were used in ambulances employed servicing in the Qom Province Medical Emergency Response Center of Iran is high, that might have their roots in non-application of disinfectants for disinfecting ambulance surfaces and equipment. This is a mandatory for healthcare agents to utilize the protective covers, especially medical gloves. Keywords: Bacterial Infections Ambulance Emergency Medical Services Qom, Iran. Please cite this article as: Farhadloo R, Shojaei S, Goodarzi Fard J, Azadeh MR, Parvaresh Masoud M, Shori Bidgoli A, Vahedian M. Evaluation of Bacterial Contamination on Pre Hospital Ambulances in Qom University of Medical Sciences of Iran in 2015. Arch Hyg Sci 2016;5(3):192-98. Background Evaluation of Bacterial Contamination on Pre Hospital Ambulances in Qom University of Medical Sciences of Iran in 2015