4067 BOOK REVIEWS Development 136 (24) The Phylogenetic Handbook: A Practical Approach to Phylogenetic Analysis and Hypothesis Testing, Second Edition Edited by Philippe Lemey, Marco Salemi and Anne-Mieke Vandamme Cambridge University Press (2009) 723 pages ISBN 978-0-521-73071-6 (paperback), 978-0-521- 87710-7 (hardback) £29.99/$59 (paperback), £70/$130 (hardback) Developmental genetic studies frequently include some form of comparative analysis; perhaps an amino acid alignment to identify conserved domains encoded within your gene of interest, or a gene tree to assess orthology or paralogy relationships with other members of a gene family. Developmental evolutionary (evo-devo) studies usually also include these sorts of analysis, but might also include assessments of character evolution across phylogenies, or patterns of molecular evolution across gene trees. Therefore, for many developmental or developmental evolutionary biologists, being able to understand and apply phylogenetic methods is an essential skill. However, the reality is that many people come to the task as novices, and that it is generally very difficult to keep pace with the ever-expanding fields of phylogenetic and molecular evolutionary analyses. Wouldn’t it be great if there were a user-friendly handbook available that kept pace with this field? Editors Philippe Lemey, Marco Salemi and Anne-Mieke Vandamme provide just that in the second edition of The Phylogenetic Handbook: A Practical Approach to Phylogenetic Analysis and Hypothesis Testing. In this edition of The Phylogenetic Handbook, the editors have taken what, in my opinion, was a decent handbook for phylogenetic analysis, namely the first edition, reorganized it and have incorporated new content, thus making the second edition a great handbook. Nearly without exception, the chapters are presented at a level that will guide both the novice and the more experienced user through what are often considered to be the ‘black boxes’ of phylogenetic and molecular evolutionary analyses. In order to facilitate an understanding of both theory and practice, each chapter is presented in two parts. First, the background and the basic theory for a particular type of data analysis (e.g. multiple sequence alignment, phylogenetic inference using maximum likelihood methods, natural selection and adaptation of molecular sequences) are presented. Each theory section is then followed by a practice section, which walks the reader through phylogenetic or molecular evolutionary analyses using examples of software packages and data sets. These are easily accessed and downloaded through links on the book’s website (http://www.thephylogenetichandbook.org). Despite the fact that this is an edited volume, which could easily lead to inconsistencies between chapters in terms of the clarity and the accessibility to the non-expert reader, the theory sections of the handbook are, nearly without exception, highly informative and should be accessible to a novice user of phylogenetic methods. The practice sections of each chapter are, however, what truly make this an eminently useful and user-friendly handbook. In each practice section, readers receive step-by-step instructions that allow them to take sample data sets through a number of phylogenetic and molecular evolutionary analyses, using selected software packages. Importantly, the reader is instructed and warned about critical model and parameter choices that must be made when executing analyses, and usually the reader is referred back to the theory section to ensure that informed, as opposed to ‘black box’, choices are made. A further plus is that some chapters also provide information on how to use newer tree-viewing software that aids the transition from phylogenetic analysis to publication-quality tree images. The new, much expanded second edition of The Phylogenetic Handbook contains 21 chapters organized into eight thematic areas. By comparison, the first edition contained 15 chapters that were presented without higher- level organization. The themes range from data preparation and the use of sequence databases; to multiple sequence alignment and phylogenetic inference (including distance, parsimony, maximum likelihood and Bayesian criteria); to the testing of models, including models of molecular evolution, molecular clock, and tree topologies; to the use of codon-based models of evolution for detecting natural selection/adaptation of molecular sequences. The additional content included in the second edition reflects the major advances in the field that have been made over the past ten years. Of most importance for developmental and developmental evolutionary biologists will be the new and/or expanded chapters that cover multiple sequence alignments, Bayesian phylogenetic analysis, the selection of models of evolution for phylogenetic analyses, the testing of alternative tree topologies, and the detection of a history of natural selection/adaptation using codon- based models of evolution. Even if you own a copy of the first edition, you should still consider replacing it with the second edition because it covers important advances in the field that have taken place since the publication of the first edition. In addition to the book itself, the accompanying website (www.thephylogenetic handbook.org) provides some additional useful information. For example, Table 3.1 from the text can be found on the website, which lists software used for multiple sequence alignment, together with references and links to websites where the software can be downloaded. In fact, for each of the software packages discussed in the handbook, the website provides links to specific sites where it can be downloaded. All the sample data sets provided for the practice sections of the handbook are also easily downloaded from the website, and the website contains an errata page where changes to the availability of software (i.e. new URLs) that have occurred since the publication of the handbook can be found. The website will presumably be kept updated, which should allow this edition of the handbook to stay on top of further progress in the field for some time. Penetrating the black box of phylogenetic analysis Lena C. Hileman University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66049, USA. lhileman@ku.edu doi:10.1242/dev.038182 Wouldn’t it be great if there were a user-friendly handbook available that kept pace with this field? [The] editors provide just that DEVELOPMENT