Asian Journal of Applied Sciences (ISSN: 2321 – 0893) Volume 02 – Issue 03, June 2014 Asian Online Journals (www.ajouronline.com) 323 Tablet Formulation of The Ethyl Acetate Soluble Extract of Soursop (Annona muricata L.) Leaves Yetri Elisya 1 , Leonardus B.S. Kardono 2 and Partomuan Simanjuntak 3 1 Polytechnic of Health Jakarta II Polytechnic, Department of Pharmacy. Indonesia Email : yetri.elisya@yahoo.co.id 2 Indonesian Institute of Sciences Indonesian Institute of Sciences, Research Center for Chemistry, Indonesia. Email : l_kardono@yahoo.com 3 Indonesian Institute of Sciences Indonesian Institute of Sciences, Research Center for Biotechnology. Indonesia. Email : partomsimanjtk@yahoo.com _________________________________________________________________________________________________ ABSTRACT--- Tablet dosage formulations of the soursop extract containing acetogenins active substances were prepared for adjuvant cancer therapy.This study consisted of the preparation of the extracts and cytotoxic activity evaluation, followed by the tablet formulation. Detection of the acetogenin constituents was judged by LCMS, and the cytotoxicity assays were performed using BSLT, and the MTT assays. BSLT test using larvae shrimp (Artemia salina Leach) aged 48 hours and MTT was performed at the Center for Primate Studies, Institute for Research and Community Service Institute of Agriculture (IPB), Bogor, Indonesia. The antioxidant activity and the stability evaluation of the formula were also observed. LCMS test soursop leaf ethyl acetate fraction showed a wide acetogenin obtained. Initial screening toxicity test with BSLT showed cytotoxic response of highly toxic ethyl acetate fraction with LC 50 = 12.188 ppm, whereas in vitro by MTT assay test showed IC 50 = 815.922 ppm. IC 50 determination of antioxidant activity by DPPH IC 50 results soursop leaf ethyl acetate fraction 94.169 ppm. Tablet F1 - F4 with a dose of 1-20 mg produced tablets with good quality test. Stability test was referring to the determination of total acetogenin which is also a marker compounds by spectrophotometric method and calculated Arrhenius equation shows that the age of the tablet product was 2.06 years. Tablets containing acetogenins of soursop leaves ethyl acetate-soluble fraction can be prepared by wet granulation and expected to have benefit for traditional cancer adjuvant therapy. Keywords---- ethyl acetate fraction of the leaves of soursop, Annona muricata L., antioxidant activity, adjuvant anticancer therapy, tablets. _________________________________________________________________________________________________ 1. INTRODUCTION Annona muricata L. (Annonaceae) is grown commercially as a fruit crop and is called “guanabana”, “graviola” or “sour sop” throughout the tropical regions of the world. It has reported to have a number of monotetrahydrofuran (mono-THF) acetogenins from the bark, seeds, and leaves. The annohexocin and murihexocins A and B were hexahydroxylated mono-THF ring acetogenins, and the annomuricins A, B, and C, muricatocins A, B, and C, and annopentocins A, B, and C were pentahydroxylated mono-THF acetogenins. Annomutacin and cis- and transannomuricin- D-ones were tetrahydroxylated mono-THF acetogenins, and cis- and trans-10-R-annonacin-Aones were trihydroxylated mono-THF compounds (Kim, 1997). The classic extraction of acetogenins from plants were carried out by successive solvent extractions with increasingly polar solvents, or by liquid/liquid partition from an initial alcoholic extract (Bermejo, 2005). The Annonaceous acetogenins were readily soluble in most organic solvents. Ethanol extraction of the dried plant material followed by solvent partitions, to concentrate the compounds, was the method of choice (Alali, 1999). Major contribution to the detection, isolation, characterization, and dereplication of Annonaceous acetogenins in the last two years has come through the application of liquid chromatography-electrospray ionization mass spectrometry (LC/EIMS) techniques. Using the positive-ion mode and under conditions of atmospheric pressure in-source collision-induced dissociation (APICID), the acetogenins have provided reproducibly characteristic ion patterns and fragment ions. Analyzing the selected ion chromatograms (SIC), the presence of these acetogenins and other derivatives in