Global Advanced Research Journal of Agricultural Science (ISSN: 2315-5094) Vol. 7(6) pp. 176-182, June, 2018 Issue. Available online http://garj.org/garjas/home Copyright © 2018 Global Advanced Research Journals Full Length Research Paper Nanoparticles and antifungal activity of iron sulfide synthesized by green route using Uncaria tomentosa leaves extract Nidá M. Salem 1 , Fatin M. Abedalaziz 2 and Akl M. Awwad 1,2* 1 Department of Plant Protection, School of Agriculture, the University of Jordan, Amman, Jordan 2 Department of Materials Science, Royal Scientific Society, Amman, Jordan Accepted 18 January, 2018 This research paper described the synthesis of iron sulfide nanoparticles from iron nitrate and sodium sulfide in the presence of Uncaria tomentosa leaves aqueous extract at ambient temperature. The iron sulfide nanoparticles (FeSNPs) were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS), UV-visible spectroscopy (UV-vis) and Fourier transform infrared spectroscopy (FT-IR) techniques. The synthesized FeSNPs were found to be spherical in shape with average size diameter 40nm. Iron sulfide nanoparticles exhibited strong antifungal activity towards plant pathogenic fungus, Fusarium oxysporum f.sp. lycopersici. These results led us to extend our research to study the effect of FeSNPs on the control of Fusarium wilt in tomato crops as fungicide and plant growth. Keywords: iron sulfide nanoparticles • Uncaria tomentosa leaves extract• Antifungal activity • Fusarium oxysporum INTRODUCTION The present study was designed to synthesize iron sulfide nanoparticles (FeSNPs) by facile green method from sodium sulfide nonahydrate (Na 2 S.9H 2 O) and iron nitrate nonahydrate [Fe(NO 3 ) 3 .9H 2 O]in the presence of U. tomentosa leaves aqueous extract at ambient temperature. Different routes and methods have been developed to synthesize iron sulfides nanoparticles, such as solvothermal process (Kar and Chaudhuri 2005; Zhao et al., 2009). polyol mediated process (Ivantsov et al., 2017), microbial synthesis (Kim et al., 2015; Zhou et al., 2017), hydrothermal synthesis (Pua et al., 2010; Akhoondi et al., *Corresponding Author’s Email: akl.awwad@yahoo.com 2013), sulfurization of hematite nanowires (Cummins et al., 2013), chemical precipitation method (Kim et al., 2011; Liu et al., 2017), a single source precursor approach (Zhang et al., 2010), chemical bath deposition (Akhtar et al., 2015), high-energy mechanical milling and mechanochemical processing (Chin et al., 2005), microwave synthesis (Xiao et al, 2016), from dithiocarboxylate precursor complex decomposition (Maji et al., 2012), from natural pyrite (Ding et al., 2013). In this study, we report for the first time a novel, rapid, cost- effective and environmentally biosynthesis of iron sulfide nanoparticles using U. tomentosa leaves extract at ambient temperature. This biosynthetic green route for iron sulfide nanoparticles was found to be extremely effective against