_____________________________________________________________________________________________________ *Corresponding author: E-mail: mkshakya2020@gmail.com, mkshkaya2020@gmail.com; Journal of Pharmaceutical Research International 33(40A): 35-43, 2021; Article no.JPRI.71762 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Evaluation of Antiepileptic Effect of Cleome viscosa Linn. Leaves Extract in Experimental Animals Manish Kumar Shakya 1* , Arif Naseer 1 and Ranjit Singh 1 1 Department of Pharmacology, Adarsh Vijendra Institute of Pharmaceutical sciences, Shobhit University, Gangoh, Saharanpur-247341, U.P, India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i40A32217 Editor(s): (1) Dr. Rafik Karaman, Al-Quds University, Palestine. Reviewers: (1) Malinee Pongsavee, Thammasat University, Thailand. (2) Rizaldy Pinzon, Universitas Kristen Duta Wacana, Indonesia. Complete Peer review History: https://www.sdiarticle4.com/review-history/71762 Received 25 May 2021 Accepted 31 July 2021 Published 04 August 2021 ABSTRACT Aim: The purpose of this study was to assess the antiepileptic effect of Cleome viscosa Linn. leaves extract in experimental animals. Study Design: The extraction process, acute toxicity study was determined using OECD guidelines, Priliminary phytochemical screening, Antiepileptic pharmacological screening methods and statistical analysis. Place and Duration of Study: The research work was conducted during 10 Jan. 2020 to 10 July 2020 at Dept. of Pharmacology, Rajiv academy for Pharmacy, Mathura (U.P), 281001, India. Methodology: The fresh leaves were shade dried and reduced in size to powder and extracted by soxhlet apparatus. The MECV, CECV and AECV were prepared and subjected to comparative phytochemical profiling for in-vitro analysis. Further the in-vivo screening models like maximal electroshock induced seizures (MES), picrotoxin (PTX) and pentylenetetrazole (PTZ) induced models are used to assess the anti-epileptic effects of the methanol, chloroform and aqueous extracts of Cleome viscosa. Results: The extracts were subjected to phytochemical tests and the carbohydrate, tannins, alkaloids, saponins, flavonoids, steroids and glycosides were found to be present. In the MES induced seizures, MECV (200 mg/kg) showed high significant inhibition on tonic hind limb extension (THLE) (9.33±0.33 *** ), decrease in duration of stupor period (145.2 ± 2.59 *** ) and Original Research Article