~ 815 ~ Journal of Pharmacognosy and Phytochemistry 2020; 9(2): 815-818 E-ISSN: 2278-4136 P-ISSN: 2349-8234 www.phytojournal.com JPP 2020; 9(2): 815-818 Received: 09-01-2020 Accepted: 11-02-2020 Imtijungla Jamir Sam Higginbottom University of Agriculture, Technology and Sciences, Niani Road, Mahewa, Allahabad, Uttar Pradesh, India PW Ramteke Sam Higginbottom University of Agriculture, Technology and Sciences, Niani Road, Mahewa, Allahabad, Uttar Pradesh, India Vijay Bahadur Sam Higginbottom University of Agriculture, Technology and Sciences, Niani Road, Mahewa, Allahabad, Uttar Pradesh, India PK Shukla Sam Higginbottom University of Agriculture, Technology and Sciences, Niani Road, Mahewa, Allahabad, Uttar Pradesh, India Corresponding Author: Imtijungla Jamir Sam Higginbottom University of Agriculture, Technology and Sciences, Niani Road, Mahewa, Allahabad, Uttar Pradesh, India Influence of photosynthetic bacteria and biochar on growth, yield and biochemical parameters of broccoli ( Brassica oleracea var. italica ) Imtijungla Jamir, PW Ramteke, Vijay Bahadur and PK Shukla Abstract The present experiment was conducted to determine the influence of photosynthetic bacteria and biochar on growth, yield and biochemical parameters of broccoli (Brassica oleracea var. italica) in the Department of Horticulture, Allahabad School of Agriculture, SHUATS, Allahabad (U.P) during the year 2017-2018. Ten treatments were included in the trial were viz; T0 Control, T1 (PSB 50L/ha + Biochar 20t/ha), T2 (PSB 100L/ha + Biochar 30t/ha), T3 (PSB 150L/ha + Biochar 50t/ha), T4 (PSB 50L/ha + Biochar 30t/ha), T5 (PSB 100L/ha + Biochar 50t/ha), T6 (PSB 150L/ha + Biochar 20t/ha), T7 (PSB 50L/ha +Biochar 50t/ha), T8 (PSB 100L/ha + Biochar 20t/ha), T9 (PSB 150L/ha+ Biochar 30t/ha) and were tested in three replications. The experiment was laid out in simple R.B.D. Studies showed that, significant effect on the height of plant 90 DAT (65.83 cm), number of leaves 90 DAT (32.47), days to first visible curd (71.07 days), leaf area 90 DAT (588.09 cm 2 ), leaf width 90DAT (20.42 cm), fresh weight of plant (995.75 g), fresh root weight of plant (105.5 g), dry weight of plant (193.6 g), dry root weight (39.47 g), head yield per plot (4.49 kg), bud or head weight (499.37 g), bud or head diameter (16.55 cm), head yield per hectare (14.98 t/ha), chlorophyll ‘a’ (1.74 mg/g/Fr.wt.), chlorophyll ‘b’ (1.59 mg/g/Fr.wt.), total chlorophyll (3.59 mg/g/Fr.wt.), total carotenoid (1.12 mg/g/Fr.wt.), leaf proline content (0.011 mg/g/Fr.wt.), vitamin C (mg/100g broccoli fresh head) (91.55 mg), gross return (Rs 374,500/ha), net return (Rs.260,050/ha), Benefit Cost ratio (3.27:1) was observed maximum in treatment T2 (PSB 100L/ha + Biochar 30t/ha). Keywords: broccoli, PSB, biochar, yield, growth, biochemical Introduction Broccoli (Brassica oleracea var. italica) belongs to family Brassicacea. It is an edible green plant in the cabbage family whose large flowering head is eaten as a vegetable. The word broccoli comes from the Italian plural of broccoli, which means "the flowering crest of a cabbage", and is the diminutive form of broccoli, meaning "small nail" or "sprout". The optimum temperature range of 10-25 "C is ideal for proper growth of the crop. The nutritive value of broccoli per 100g is energy 141kj, carbohydrates 6.64g, sugar 1.7g, dietary fiber 2.6g, fat 0.37g, protein 2.82g, vit A 9000IU, calcium 47mg, iron 0.73mg, phosphorous 66mg, thiamine 0.071mg, riboflavin 0.117mg, niacin 0.639mg, vit C 89.2mg. Broccoli Green magic (F1) hybrid plant produces medium size blue-green broccoli. The head averages 6 to 9 inches in diameter. It is very flavorful. This broccoli has very good uniformity and holding ability. This heat tolerant variety can be grown for summer or fall harvest. A cool-weather crop that can be grown in spring and fall everywhere, and even in winter in mild climates. In addition to its economic importance, Epidemiological studies have shown that consumption of Brassica vegetables, including broccoli, reduces the risk of several types of cancers and cardiovascular disease mortality. The excessive use of chemical fertilizers has caused tremendous harm to environment. So nowadays, the pursuit of quality, fresh, non-toxic and safe has become the trend of global agricultural production. Application of photosynthetic bacteria and biochar in farming is regarded as environmental friendly, and can be used to reduce excessive amount of chemical fertilizer application, and ensures a sustainable crop production. Photosynthetic bacteria, much like the name suggests, these micro-organisms are special types of bacteria that contain light absorbing pigments and reaction centers which make them capable of converting light energy into chemical energy. Photosynthetic bacteria (PSB) have been extensively used in agriculture to promote plant growth and to improve crop quality. Biochar is Charcoal used as a Soil conditioner or soil enhancer to increase fertility, prevent soil degradation. It improves soil fertility by retaining water and nutrients in soil, encouraging beneficial soil organisms and