Research Journal of Life Science APRIL-2020 Volume 7 NO. 1 (52-61) journal homepage: www.rjls.ub.ac.id How to cite this article: Ventiyaningsih, A. D. I., Sujuti, H., Nurtika, W., Rachmawati, S. N., Nurshalillah, Jannah, R. (2020). The Effect of Moringa Oleifera Leaves Powder to Level of Serum Superoxide Dismutase (SOD), Lead (Pb), Zink (Zn) and Memory Function of Rat (Rattus norvegicus) Wistar Strain Model of Autism that is Exposed by Pb. Research Journal of Life Science, 7(1), 52-61. https://doi.org/10.21776/ub.rjls.2020.007.01.6 The Effect of Moringa Oleifera Leaves Powder to Level of Serum Superoxide Dismutase (SOD), Lead (Pb), Zink (Zn) and Memory Function of Rat (Rattus norvegicus) Wistar Strain Model of Autism that is Exposed by Pb Agustiana Dwi Indiah Ventiyaningsih 1* , Hidayat Sujuti 2 , Winda Nurtika 1 , Septi Nur Rachmawati 1 , Nurshalilah 1 , Raudhatul Jannah 1 1 Department of Nutrition, Faculty of Medicine, University of Brawijaya 2 Laboratory of Biochemistry, Faculty of Medicine, University of Brawijaya Email address: agustiananventy@ub.ac.id Abstract Lead exposure (Pb) may aggravate the decrease in serum SOD levels, memory function reduction and social interaction in autism. This study aims to determine the effect of Moringa oleifera on autism models of rat exposed to lead. The five groups of rat exposed to tin were studied: normal rat (C -), autistic rat (C +) and autism rats given 180 mg (T1), 360 mg (T2) or 720 mg (T3) oleifera leaves powder. Lead acetate is given through a filler tube (0.5 gr / kg) and powdered moringa leaves mixed with feed. The results showed that serum SOD levels were lower in autistic rat than normal rat and intake of Moringa olifeifera leaves powder increased serum SOD levels in autistic rats but was not seen in normal rat. The autistic rat had higher lead levels but lower zinc levels, which were not influenced by Moringa olifeira. The social interaction between rats is strongly influenced by unbidden lead with Moringa olifeira, both in normal rat and in autistic rat. Moringa oleifera will increase serum SOD levels in autistic rats but not at normal levels. Moringa olifeira does not protect significantly rat from lead causing less social interaction, nor does it lower serum Pb levels or increase Zn levels. Moringa leaves powder does not affect memory significantly. Introduction Autism is a neurological disorder with limitations on social interaction, communication, hyperactive behavior, and intellectual (American Psychiatric, 2013). The prevalence of autism increases annually with an increase of 0.84% in 2012 to 2% in 2007 (Blumberg et al., 2013). Based on the results of the US Census Bureau in 2005, people with autism in Indonesia reached 475,000 people (Mahardhika & Budiono, 2012). The risk of autism is five times higher in boys (Christensen et al., 2016). Combined internal and external factors lead to greater risk of autism. Impaired regulation of neurological function due to brain abnormalities (Rahayu, 2014), genes (Chaste & Leboyer, 2012), and damage to the amygdala can cause disruption to the delivery of information to the brain, impaired expression and emotion (Schultz, 2005). They cause fear, anxiety, and social behavior disorders (Wang et al., 2013). Previous research has shown that the distribution of neuronal disorders in sodium valproate-induced rats (NaVPA) causes autism symptoms such as limited social interactions (Wang et al., 2013) (Chen et al., 2014) and hyperactivity (Markram et al., 2008). The state of autism can be exacerbated by heavy metal contamination. This is because KEYWORDS Autism Memory Moringa Oleifera Pb Zn Social Interaction SOD