Research Artcle Copyright (c) 2019 Journal of Advanced Research in BioChemistry and Pharmacology htps://www.adrpublicatons.in Journal of Advanced Research in BioChemistry and Pharmacology Volume 2, Issue 1 - 2019, Pg. No. 11-17 Peer Reviewed & Open Access Journal Corresponding Author: Hatl Hashim EL-Kamali, Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan. E-mail Id: hatlhashim@gmail.com Orcid Id: htps://orcid.org/0000-0002-9210-7993 How to cite this artcle: Hatl Hashim EL-Kamali, Nadia Nour El-Din Abd- AL-Gadir, Ramadan MM et al. Water Extract of Three Aromatc Plants Mixture Ameliorates Paracetamol-Induced Renal-Hepato Damage in Male Albino Rats. J Adv Res BioChem Pharma 2019; 2(1): 11-17. Date of Submission: 2019-01-16 Date of Acceptance: 2019-03-16 I N F O A B S T R A C T Water Extract of Three Aromatic Plants Mixture Ameliorates Paracetamol-Induced Renal-Hepato Damage in Male Albino Rats Hatil Hashim EL-Kamali 1 , Nadia Nour El-Din Abd-AL-Gadir 2 , Manal Mohammed Ramadan 3 , Kadry Z. Ghanem 4 , Abdel Razik H. Farrag 5 1,2 Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan. 3 Department of Chemistry of Flavour and Aroma, Natonal Research Centre, Dokki, Giza, Egypt. 4 Department of Clinical Nutriton, Faculty of Applied Medical Science, Jazan University, KSA. 5 Department of Pathology, Natonal Research Centre, Dokki, Giza, Egypt. Background and Objectves: This study examined the efects of fruit water extracts of mixture from selected three plants (Xylopia aethiopica, Coriandrum satvum and Anethum graveolens) on some indices of liver and kidney functon tests in the male albino rats, and also to evaluate the antoxidant power of its water extract and the protectve efect against paracetamol-induced hepatc and renal toxicity in male albino rats. Materials and Methods: Three groups of rats were used (control, paracetamol-treated and protected group), which were supplemented with mixture water extract for four weeks followed by intraperitoneal injecton of paracetamol. Levels of ALT, AST, ALP, gamma-glutamyl transferase, CAT, GP x , serum direct bilirubin, serum total bilirubin, serum albumin, serum total globin, total serum urea and serum creatnine, as well as histopathological changes in the liver and kidney were investgated. Quanttatve determinaton of the total phenolic content (TPC) was performed using the Folin-Ciocalteu method, quanttatve determinaton of antoxidant actvity was performed according to the β-carotene bleaching method and Diphenylpicrylhydrazyl (DPPH) free radical scavenging assay. The experiment was conducted for two weeks. Statstcal analysis was carried out by analysis of variance. Results: Oral administraton of paracetamol recorded signifcant decrease in TAC, CAT and GPx compared to normal control rats whereas rats supplemented with water extract and then intoxicated with paracetamol showed a signifcant increase in TAC, CAT and GPx levels compared with paracetamol intoxicated rats, and compared to normal control. AST, ALT, ALP, GGT, total bilirubin, direct bilirubin and indirect bilirubin levels of rats treated with paracetamol were quite higher than that of control group whereas the rats treated with mixture extract and paracetamol had signifcant reducton when compared with the paracetamol group. Paracetamol induced signifcant increase in the concentraton of plasma urea and creatnine in intoxicated rats as compared to normal control rats, whreas there was no signifcant diference between mixtures