Buffalo Bulletin (March 2015) Vol.34 No.1 63 ABSTRACT The experiment was designed to provide higher plane of nutrition, vitamin E and mineral supplementation for augmenting the improvement in reproductive performance. In the present investigation, 10 Murrah buffaloes each in two groups, expected to calve in winter season were selected during prepartum period. None of the buffaloes during periparturient period suffered from any clinical metabolic disease or reproductive disorders. Plasma Ca and Plasma inorganic P concentration showed significant difference on day 15 prepartum (P<0.01); Zn on day 15 prepartum (P<0.05) and day 15 postpartum (P<0.01); Cu on day 30 prepartum (P<0.05) and Mn on day 30 prepartum (P<0.05), however, the differences in concentrations at all other stages were non-significant but the supplemented group had higher levels than the control group at all the stages. The concentration of the plasma glucose exhibited significant difference (P<0.01) at 45 days postpartum but the supplemented group had higher levels than the control group at all the stages. The plasma BUN showed significant difference (P<0.05) at days 30 and 15 prepartum, calving day and on day 30 postpartum (P<0.01) and Plasma NEFA and vitamin E values obtained were statistically not significant. However, the supplemented group had lower levels than the control group at all the stages but vitamin E values had higher levels than the control group at all the stages. Cervical and uterine involution was completed in lesser days, involutory changes took place at a faster pace and there were lesser percent of cows suffering from abnormal uterine changes in supplemented compared to control group. Supplemented group showed better reproductive performance considered in the study than control group. In total, around 12 days could be saved in days to first service if vitamin E and minerals were supplemented. Supplemented group showed early initiation of cyclicity (32 days postpartum) compared to control group (35 days postpartum). Cyclicity in most of the animals might have been initiated earlier than 30days as was evident from progesterone concentration (>1 ng/ml). Short and long luteal phases were observed on appraisal of progesterone concentration in both the groups which delayed the days to first service in these animals. It can be concluded that mineral and vitamin E supplementation improved the reproductive performance of buffalo during periparturient period. EFFECT OF VITAMIN E AND MINERAL SUPPLEMENTATION ON BIOCHEMICAL PROFILE AND REPRODUCTIVE PERFORMANCE OF BUFFALOES 1 Sheep Research Station, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), E-mail: hilal.ndri@gmail.com, bhakat. mukesh@gmail.com 2 Artificial Breeding Research Centre, National Dairy Research Institute, Karnal, Haryana, India 3 Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal, Haryana, India H.M. Khan 1 , T.K. Mohanty 2 , M. Bhakat 2 , A.K. Gupta 2 , A.K. Tyagi 3 and G. Mondal 3 Original Article