~ 155 ~ Journal of Pharmacognosy and Phytochemistry 2014; 3(3): 155-159 E: ISSN 2278-4136 P: ISSN 2349-8234 JPP 2014; 3(3): 155-159 Received: 07-08-2014 Accepted: 30-08-2014 Vishakh R Research Scholar, K. S. Hegde Medical Academy, Nitte University, Deralakatte, Mangalore. Shailaja Moodithaya S Associate Professor, Dept. of Physiology, K. S. Hegde Medical Academy, Nitte University Deralakatte, Mangalore. Suchetha Kumari N Professor, Dept. of Biochemistry, K. S. Hegde Medical Academy, Nitte University, Deralakatte, Mangalore. Correspondence: Vishakh R Research Scholar, K. S. Hegde Medical Academy, Nitte University, Deralakatte, Mangalore. Evaluation of antioxidant property of West Coast Tall variety tender coconut water and synthetic Trans – Zeatin – An in-vitro study Vishakh R, Shailaja Moodithaya S and Suchetha Kumari N Abstract Background: Coconut (Cocos nucifera L.) has been described as the most important and extensively grown palm tree worldwide and had been widely used for human consumption in tropical countries because of its nutritional properties and low cost. Various studies had proved Cocos nucifera water was found to be effective in maintain electrolytic balance in body. trans-Zeatin on other hand is a cytokine abundantly found in TCW had shown significant anti-ageing, anti-carcinogenic, and anti-thrombotic effects. Materials and methods: Phytochemical analysis, Total phenolic content, Free Radical Scavenging by DPPH Assay and ABTS assay, FRAP Assay and Total antioxidant capacity of west coast tall variety TCW (West Coast Tall Variety) 3 to 4 months old .was done using standard methods. Results and Conclusion: Phytochemical analysis of West coast tall variety TCW (TCW) showed the presence of Carbohydrates, Proteins, Flavonoids, Terpenoids, Glycosides, Phenolic Compounds and Steroids. In vitro study also proved that the TCW is a rich source of antioxidants, both TCW and synthetic trans-Zeatin exhibits antioxidant activity by exhibiting its free radical scavenging potential. TCW is a rich source of phenolic compounds. The study concludes that the effective free radical scavenging ability of TCW might be attributed due the presence of phenolic compounds. Keywords: Phytochemicals, Phenolic compounds, Free radicals, trans-Zeatin, Tender Coconut water 1. Introduction Coconut (Cocos nucifera L.) has been described as the most important and extensively grown palm tree all over the world. It belongs to the family Arecaceae. Traditionally TCW was used as an emergency glucose supply in absence of sterile glucose. It was used as intravenous fluid to counteract the effect of drug overdose, poisoning and adverse drug reaction, extensively used as the refreshment drink after physical exercise [1] . Till date it has been known that TCW(TCW) is a good source of various biomolecules like Sugar, Vitamins, Electrolytes, Plant hormones like Zeatin, Kinetin etc., [2] Various studies have proved that the cytokines present in tender coconut, in addition to various plant-related functions also showed significant anti-ageing, anti-carcinogenic, and anti-thrombotic effects [3, 4] . It has been reported that the coconut water is useful in conditions like digestive disturbances, kidney stones, urinary tract infections, and sterility [4] . Zeatin, one of the important class of the phytohormone present abundantly in the TCW has been extensively used in plant tissue culturing. It has also been proved to be a potential agent for the management of skin photo aging [5] . Studies suggests that zeatin might be useful for protecting cognitive dysfunction, as well as for reducing the activation of Acetyl Choline Esterase in dementia [6] . Free radicals are known to play a definite role in a wide variety of pathological manifestations of pain, inflammation, cancer, diabetes, alzheimer, hepatic damage etc. Antioxidants fight against these free radicals either by Scavenging the reactive oxygen species or protecting the defense mechanisms [6] and protect us from various diseases [7] . Various studies have been done to evaluate the composition of coconut water, but less research has been done to know the composition of Tender Coconut in costal belt of Karnataka. Therefore the current study aimed to evaluate the phytochemicals components present in TCW and the in-vitro Further no significant work is done to evaluate the properties of trans-Zeatin which is the important phytohormone present in TCW. Antioxidant properties of both TCW