Analytical Chemistry, 2016 | S- 1 Supporting Information Quantitative determination and subcellular imaging of Cu in single cells via laser ablation-ICP-mass spectrometry using high-density microarray gelatin standards Stijn J.M. Van Malderen 1 , Eva Vergucht 1 , Laszlo Vincze 1 , Maarten De Rijcke 2 , Colin Janssen 2 , Frank Vanhaecke 1* 1 Department of Analytical Chemistry, Ghent University, Campus Sterre, Krijgslaan 281 - S12, 9000 Ghent, Belgium 2 Department of Applied Ecology and Environmental Biology, Ghent University, Jozef Plateaustraat 22, 9000 Ghent, Belgium * Corresponding author. E-mail address: Frank.Vanhaecke@UGent.be Table of contents  Cell culture conditions S-2 Describes the conditions in which the microalgae were captured and grown.  Microwell preparation S-2 Describes the microwell preparation and related considerations.  Surface modification S-2 Describes the substrate surface modifications  Surface hydrophobicity S-2 Describes a substrate surface modification to improve the self-dispensing properties of the microarrays  Figures S-3,4,5 Additional figures (S1-S8) with descriptive annotations.