_____________________________________________________________________________________________________ *Corresponding author: E-mail: bismillah.mubeen@gmail.com, saim1692@gmail.com; Journal of Pharmaceutical Research International 33(40B): 126-138, 2021; Article no.JPRI.71058 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Enrichment of Therapeutically Significant Flavonolignans of Silybum Marianum in Vegetative Parts by Applying Fungal Elicitors, Methyl Jasmonate and Silver Nanoparticles as Elicitor in Hydroponic Culture Bismillah Mubeen 1* , Qurban Ali 1* , Ammarah Hasnain 1 and Arif Malik 1 1 Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i40B32272 Editor(s): (1) Dr. Rafik Karaman, Al-Quds University, Palestine. Reviewers: (1) Papia Haque, University of Dhaka, Bangladesh. (2) Porntip Wongkaew, Khon Kaen University, Thailand. Complete Peer review History: https://www.sdiarticle4.com/review-history/71058 Received 15 May 2021 Accepted 20 July 2021 Published 10 August 2021 ABSTRACT Introduction: Medicinal plants are being used to treat several diseases for many decades and this is an ancient method to treat the patients. Herbal plant Silybum marianum found most effective one to cure liver disorders. This plant produces silymarin which is a secondary metabolite and have hepatoprotective properties. Silymarin is a mixture of flavonolignans (silybin A, silybin B, isosilybin A isosilybin B, silychristin, silydianin apigenin 7-D glucose and taxifolin) that has antiviral, antibacterial, antifungal and antiallergenic properties. Therefore, the excessive production of silymarin is necessary to cure different types liver disorders, so the present study executed to boost up production of Silymarin flavonolignans in vegetative parts of the Silybum marianum plant by using different elicitors. Materials and Methods: In the present study, elicitation technique in hydroponics system was used to enhance the production of pharmacologically active flavonolignans in Silybum marianum. Fungal elicitors prepared from lyophilized Aspergillus niger biomass, methyl jasmonate, silver Original Research Article