Received: 11 September 2021 Revised: 20 September 2021 Accepted: 21 September 2021 DOI: 10.1002/ndr2.12040 NEW DISEASE REPORT First report of Tomato brown rugose fruit virus in greenhouse tomato in Iran A. Ghorbani 1 M. Rostami 2 S. Seifi 3 K. Izadpanah 1 1 Plant Virology Research Centre 71441-13131, College of Agriculture, Shiraz University, Shiraz, Iran 2 Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz, Iran 3 Department of Agriculture, Payame Noor University, Tehran, Iran Correspondence A. Ghorbani, Plant Virology Research Centre 71441-13131, College of Agriculture, Shiraz University, Shiraz, Iran. Email: Abozar.ghorbani@shirazu.ac.ir In August 2021, symptoms of a viral-like infection were observed on the leaves and fruits of tomato plants, cvs. Emperador RZF1 (Rijk Zwaan, The Netherlands) Maxifort, Eshkol and 4129 (Seminis Veg- etable Seeds, USA) in a 3.2 ha greenhouse complex in Isfahan province, Iran. Plants had been raised in Iran from imported seed. The symptoms consisted of severe mosaic and deformation of leaves (especially the upper and young leaves) and chlorotic and brown necrotic spots on the fruits (Figure 1). These symptoms resembled those of Tomato brown rugose fruit virus (ToBRFV) reported elsewhere (Salem et al., 2016). About 30% of plants were affected by symptoms across the green- FIGURE 1 Tomato brown rugose fruit virus symptoms on tomato leaves and fruit This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2021 The Authors. New Disease Reports published by British Society for Plant Pathology and John Wiley & Sons house complex. The cultivars Eshkol and 4129 were more severely affected than others. Extracts from leaf and fruit samples induced the formation of necrotic local lesions on mechanically inoculated Nicotiana glutinosa (Figure 2) indicating the possible presence of a tobamovirus. The sam- ples were also subjected to RT-PCR using ToBRFV-specific primers: ToBRFV-F (5’-GAAGTCCCGATGTCTGTAAGG-3’) and ToBRFV-R (5’- GTGCCTACGGATGTGTATGA-3’) (Ling et al., 2019). The fragment obtained from the PCR reaction (842 bp) was isolated, purified after size confirmation (by comparison with molecular weight markers), and New Dis Rep. 2021;44:e12040. wileyonlinelibrary.com/journal/ndr2 1 of 2 https://doi.org/10.1002/ndr2.12040