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Journal of Entomology and Zoology Studies 2015; 3(4): 331-333
E-ISSN: 2320-7078
P-ISSN: 2349-6800
JEZS 2015; 3(4): 331-333
© 2015 JEZS
Received: 22-06-2015
Accepted: 24-07-2015
Imtiaz Ali Khan
Department of Entomology,
The University of Agriculture,
Peshawar.
Hameed Khan
Department of Entomology,
The University of Agriculture,
Peshawar.
Inamullah Khan
Entomology Division, Nuclear
Institute for Food and Agriculture
(NIFA), Peshawar.
Rasheed Akbar
Department of Entomology,
The University of Agriculture,
Peshawar.
Mukhtar Alam
Faculty of Agriculture,
The University of Swabi, Pakistan.
Muhammad Saeed
Institute of Biotechnology and
Genetic Engineering,
The University of Agriculture,
Peshawar.
Abid Farid
Institute of Biotechnology and
Genetic Engineering,
The University of Agriculture,
Peshawar.
Ijaz Ali
Institute of Biotechnology and
Genetic Engineering,
The University of Agriculture,
Peshawar.
Komal Habib
Department of Entomology,
The University of Agriculture,
Peshawar.
Walija Fayaz
Department of Entomology,
The University of Agriculture,
Peshawar.
Correspondence:
Hameed Khan
Department of Entomology,
The University of Agriculture,
Peshawar.
Efficacy of some plant extracts on larval mortality
of Culex quinquefasciatus (Say) (Diptera:
Culicidae) in Peshawar
Imtiaz Ali Khan, Hameed Khan, Inamullah Khan, Rasheed Akbar,
Mukhtar Alam, Muhammad Saeed, Abid Farid, Ijaz Ali, Komal Habib,
Walija Fayaz
Abstract
Culex quinquefasciatus vectors Wuchereria bancrofti, which causes Lymphatic filariasis, a hurting and
extremely disfiguring disease, in millions of people in tropical and subtropical areas of the world each
year. Synthetic insecticides have been mainly used for the control of the insect vector. In the present
research efficacy of some plant extracts in ethanol solvent on larval mortality of C. quinquefasciatus after
24 hrs was tested at the Nuclear Institute for Food and Agriculture (NIFA), Peshawar during 2013. LC50
and LC95 values of the plant extracts were also determined. The experiment was laid out in CR Design
with 6 treatments, i.e. lemon juice, lemon grass, basil, thyme, fruit of bitter gourd and control, each
replicated three times. The plant extracts were tested each in five concentrations of 200, 300, 500, 700
and 1000 ppm. The results indicated significance differences in C. quinquefasciatus mortalities caused by
different treatments and control. Thyme and bitter gourd gave 100% mortalities of the insect at all the
concentrations in ethanol extract. LC50 value was highest for lemon juice (694.004) and LC95 value was
highest for basil (7102.726) after 24 hrs in ethanol extract. The study recommended that the plant extracts
should be tested at lower concentrations as many of the extracts yielded 100% mortalities at higher
concentrations.
Keywords: C. quinquefasciatus, Ethanol solvent extract, LC50, LC95, Mortality.
1. Introduction
Culex quinquefasciatus (Say) (Diptera: Culicidae) is a cosmopolitan mosquito species found in
tropical, subtropical, and warm temperate regions of the world. It is one of the most successful
mosquito species because it can breed in almost all type of habitats such as ponds, stagnant
water, roadside ditches, freshwater ponds, banks of rivers and natural streams, etc.
[1]
.
In Pakistan, it is found in abundance everywhere except the northern colder areas where
temperature goes below zero
o
C. In southern parts of the country diverse variety of the
mosquitoes can be found. In Khyber Pakhtunkhwa due to suitable environmental conditions
throughout the province and the ability of mosquito adaptation to diverse environmental
conditions has led it to survive everywhere
[2]
.
C. quinquefasciatus acts as a vector of Wuchereria bancrofti which causes Lymphatic
filariasis, commonly known as elephantiasis which is a hurting and extremely disfiguring
disease
[3]
. 120 million people in tropical and subtropical areas of the world suffered from
lymphatic filariasis. Among these infected people, about 25 million men suffered with genital
disease most commonly hydrocele, and 15 million (mostly women) suffered with
lymphoedema or elephantiasis of leg
[4]
. In 2001, confirmed cases of Tropical Pulmonary
Eosinophilia (TPE) were reported in indigenous patients; however the disease is rare in
Pakistan
[5]
.
Mosquito control is effective in larval stage. Larviciding is one approach to vector control,
which is carried out at breeding sites of the vectors before they emerge as adults. Larval stages
breed in water and can be more easily dealt with in this habitat; therefore, they are attractive
targets for pesticides
[6]
.
Chemical control is an effective control for pests used mostly in daily life. Synthetic pesticides
are mostly used in the world for controlling pests including mosquitoes. But there are many
draw backs of using synthetic pesticides such as these have residual effects and they also kill
non- harmful insects so most of the synthetic pesticides have environmental issues. The
mosquitoes also get resistance to insecticides with passage of time.