КСПЕРИМЕАЛНА МЕДИИНА И БИОЛОГИ EXPERIMENTAL MEDICINE AND BIOLOGY 84 Проблемы здоровья и экологии, 2020, № 3(65) Проблемы здоровья и экологии Problems of health and ecology 2020, № 3(65), с. 84–89 2020, no 3(65), pp. 84–89 ДК 615.276:616.72-018.36 ВЛИНИЕ ИНРААРИКЛРНОГО ВВЕДЕНИ ОНДРОИИНА СЛАА НА ЛЕМЕН СИНОВИАЛНОГО ССАВА В КСПЕРИМЕНЕ © В. И. НИКОЛАЕВ, Д. А. ЗИНОВКИН, А. А. РЕКОВ О «Г   », . Г, Р Б РЕЗМЕ  :              (С). М   . О     36   В. И-  С   0,05           ( ),       NaCl    ( ). Ж      12   7-, 14-  21- ,      -, -     С  0,9 % NaCl. В      ,     10 %  -  . Г   4     . П       ,         . Р . И   ,       -            2-  3-   С. П      21-           ( < 0,0001),  -         ( < 0,0001),        ( = 0,002). З . П       С, -  ,   . К : хондроитина сульфат, интраартикулярное введение, остеоартрит. В : Н В.И.:    ,    - ,    . З Д.А.:   , , -  .  А.А.:   ,     . К :      . И :     . ДЛ ИИРОВАНИ: Н ВИ., З ДА.,  АА. В          . Проблемы Здоровья и Экологии. 2020;65(3):84–89 EFFECT OF INTRA-ARTICULAR INJECTION OF CHONDROITIN SULFATE INTO SYNOVIAL JOINT ELEMENTS IN AN EXPERIMENT © VLADIMIR I. NIKOLAEV, DMITRY А. ZINOVKIN, ALEXANDER A. TRETYAKOV Gomel State Medical University, Gomel, Republic of Belarus ABSTRACT Objective: to study morphological and morphometric changes in the epiphysis bone in a rat during the intra- articular injection of chondroitin sulfate (CS). Mteril nd methods. The object of the study was the knee joints of 36 Wistar rats. CS injections at a dose of 0.05 ml were performed once a week into one of the knee joints (experimental joint), and isotonic NaCl solution at the same volume was injected into the opposite joint (control joint). The animals in the number of 12 units were withdrawn from the experiment on the 7 th , 14 th , and 21 st days, which corresponded to one week after one-, two-and three-fold intra-articular injections of HC and 0.9 % NaCl. The isolated knee joints were placed in a decalcifying liquid, then were fixed in 10% neutral buffered formalin. 4 micron histological sections were stained with hematoxylin and eosin. The morphometric analysis assessed the thickness of hyaline articular cartilage, the thickness of the epiphysis growth zone cartilage, and the cell content of the subchondral bone. Results. The study of the thickness of the articular cartilage, the growth zone of epiphyseal cartilage and the cellular composition of the subchondral bone has showed a statistically significant dynamic increase in these indica- tors after the 2 nd and 3 rd intra-articular injections of CS. The assessment of the thickness of the articular cartilage on the 21 st day found some statistically significant differences between the experimental and control groups (p < 0.0001), the thickness of the epiphyseal cartilage had increased significantly by that time (p < 0.0001), and the cell content of bone marrow showed statistically significant differences (p = 0.002). Conclusion . The obtained data testify to a pronounced regenerative effect of CS, injected intra-articularly into articular cartilage. Key words: chondroitin sulfate, intra-articular injection, osteoarthritis.