Int.J.Curr.Microbiol.App.Sci (2017) 6(7): 4305-4309 4305 Short Communication https://doi.org/10.20546/ijcmas.2017.607.447 Effect of Hemidesmus indicus Extract against Methcillin Resistant Staphylococcus aureus from Various Clinical Specimens B. Vignesh Kanna 1 , E. Kavitha 2 , M. Swapna 2 and Joshy M. Easow 3 * 1 Department of Microbiology, Annapoorana medical College and Hospital, Salem, India 2 Department of Microbiology, Sri Venkateshwaraa Medical College, Hospital and Research centre, Pondicherry, India 3 Nirmal lab, Pondicherry, India *Corresponding author ABSTRACT Introduction Human infections involving microorganisms i.e. bacteria, fungi, viruses, cause serious infections in tropical and subtropical countries of the world. In recent years, a multidrug resistance microorganism is on rise due to indiscriminate use of commercial drugs (Gutmann et al., 1988; Mohanasundari et al., 2007). Staphylococcus aureus is a Gram positive cocci arranged in clusters and is ubiquitous in nature. Penicillin was the drug of choice, to which Staphylococcus aureus developed resistance by producing enzyme beta lactamase. So, Methicillin was introduced in 1959. But, resistance to Methicillin emerged rapidly and in 1961 Methicillin resistant Staphylococcus aureus (MRSA) cases were reported (Poonam et al., 2008). Methicillin Resistant Staphylococcus aureus (MRSA) strains represent a worldwide threat because of their virulence and broad distribution in the hospital settings and International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 7 (2017) pp. 4305-4309 Journal homepage: http://www.ijcmas.com Methicillin Resistant Staphylococcus aureus (MRSA) strains represent a worldwide threat because of their virulence and broad distribution in the hospital settings and community. MRSA strains are often resistant to beta lactam antibiotics and also to flouroquinolones, chloramphenicol, clindamycin, tetracycline and aminoglycosides. In addition, resistance is shown to recently develop anti-staphylococcus agents including the Oxazolidione and Streptogramin. Increasing in multi-drug resistant strains there is a need of alternative source. Bioactive compounds from natural products and traditional medicinal plants show promising role in treatment to control MRSA infection. We aimed to report the anti MRSA activity of ethanolic extract of Hemidesmus indicus root and its possible interaction with antibiotics. A total of 100 MRSA isolates from various clinical samples were included in the study. Ethanolic root extract of Hemidesmus indicus was done by using soxhlet apparatus. These isolates were subjected to perform antimicrobial susceptibility testing by Well-diffusion method, MIC and synergistic interaction with antibiotics. In this study for the concentration of 50μl shows 25% were sensitive and 75% were resistant. At 100μl 61% were sensitive and 39% were resistant. For MIC ranging from 6.20 to 10.65mg, 70% shows sensitive and 30% were resistant. Doxycycline, ciprofloxacin and amikacin 61%, 45%, 40% were showed synergism respectively. Keywords Hemidesmus indicus, MRSA, MIC. Accepted: 29 June 2017 Available Online: 10 July 2017 Article Info