71 Vol. 52 No 4 2006 Effect of peucedanin and bergapten (5-MOP), furanocoumarins isolated from Peucedanum tauricum Bieb. (Apiaceae) fruits, on apoptosis induction and heat-shock protein expression in HeLa cells  M. BARTNIK * , K. GŁOWNIAK  , J. JAKUBOWICZ-GIL 2 , B. PAWLIKOWSKA-PAWLĘGA 2 , A. GAWRON 2  Department of Pharmacognosy with Medicinal Plant Laboratory Skubiszewski Medical University of Lublin Chodźki , 20–093 Lublin, Poland 2 Department of Comparative Anatomy and Anthropology Maria Curie-Skłodowska University Akademicka 9, 20–033 Lublin, Poland *corresponding author; e-mail: mbartnik@pharmacognosy.org Summary In the research effects of bergapten (5-MOP) and peucedanin, furanocoumarins isolated from fruits of Peucedanum tauricum Bieb. (Apiaceae), on apoptosis, necrosis and heat-shock proteins Hsp 27 and Hsp 72 expression in human carcinoma cells HeLa B (ECACC No 8506070) were preliminary studied. The purity of analysed compounds was confirmed by RP HPLC, EI-MS and D, 2D NMR. For apoptosis and necrosis detection the mixture of Hoechst 33342 and propidium iodide (PI) was used. Early apoptosis was detected using Annexin V Test. Cells were incubated with fu- ranocoumarins at the concentrations of , 5, 0 and 5 μg/ml. The level of heat-shock pro- tein expression was determined by Western blot technique. Quantitative analysis of Hsp 27 and Hsp 72 was done with the Bio-Profil Bio-D Windows Application V.99.03 program. The obtained results were analysed for significance by one-way ANOVA test. As a result of our experiments we found that peucedanin was more effective in apoptosis induction than bergapten. In HeLa cells treated with peucedanin only low necrosis was observed. The in- cubation with 5 μg/ml of peucedanin for 24 hours inhibited Hsp 72 expression in cells by 77.5% (± 0.05), and Hsp 27 by 74.0% (± 0.02) after five hours of treatment as compared to the control. The inhibition of Hsps expression after peucedanin treatment may be respon- sible for increased apoptosis induction (.4%) detected by Annexine V test. Incubation The paper was presented as a poster at the Conference “Modern Phytotherapy — Adverse Drug Effects and Interactions”, held on November 7, 2006, in Poznań.