International Journal of Research Studies in Biosciences (IJRSB) Volume 2, Issue 3, April 2014, PP 1-7 ISSN 2349-0357 (Print) & ISSN 2349-0365 (Online) www.arcjournals.org ©ARC Page | 1 Histochemical and Histopathological Observations on Pancreas Rat Treated with 1, 5-Bis (3, 5-Dimethylpyrazol-1-Yl)-3- Oxapentane-Diacetatocopper A. E. Nofal Zoology Department, Faculty of Science Menoufia University, Egypt nofal83@yahoo.com A. S. Potapov I. Polzunov Altai State Technical University, Barnaul, Russia asp99@yandex.ru A. I. Khlebnikov I. Polzunov Altai State Technical University Barnaul, Russia aikhl@chem.org.ru Abstract: The present study provides evidence that 1, 5-Bis (3, 5-Dimethylpyrazol-1-yl)-3-oxapentane- diacetatocopper has an antidiabetic effect, as hypoglycemic agent and as antilipolytic agent, but with many abnormalities. Rats treated with 1,5-Bis (3,5-Dimethylpyrazol-1-yl)-3-oxapentane-diacetatocopper for 2, 4 and 6 weeks in the present study revealed many histopathological alterations in the pancreas; inflammatory infiltration, marked enlarged vacuolated cytoplasm in pancreatic cells, congestion of blood vessels with edema and haemorrhage, as well as, some of the degenerated cells showed karyorhexis, pyknosis and area of necrosis. The present study revealed significant decrease in proteinic scontents in the pancreas. Keywords: proteinic contents, pancreas, pyknosis and necrosis. 1. INTRODUCTION The pancreas is an organ composed of endocrine and exocrine tissue. The endocrine portion is the islets of langerhans, contains cells that secrete insulin and glucagon hormones. The exocrine pancreas is a complex gland which contains many secretary acini which produce and release digestive enzymes. Insulin is hormone secreted by the isles of Langerhans in the pancreas into the blood when animals eat and the glucose level in the blood increases. Insulin stimulated the uptake, storage and use of glucose by tissues. These activities result in a decrease in the level of blood glucose (Butler, 1995). 3,5-dimethylpyrazole (DMP) as a hypoglycemic agent (one of various agents that decrease the level of glucose in the blood and are used in the treatment of diabetes mellitus) and as antilipolytic agent (one of agents that inhibits lipolysis ). 3, 5-dimethylpyrazole markedly depressed plasma fatty acid (FFA) and blood sugar after 15 minutes to 3 hours of its administration. The mechanism of hypoglycemic activity of 3, 5-dimethylpyrazole is not the same as insulin. The action 3, 5-dimethylpyrazole is similar to that of insulin in that; it increases glucose oxidation and decreases plasma free fatty acids of intact rats as reported for insulin and decrease blood glucose. 3, 5-dimethylpyrazole is unlike insulin in that; It is not effective in lowering blood sugar of eviscerated rats which respond to insulin (George and William, 1965). Hypoglycaemia and stimulation of the endocrine pancreas of rats after 3, 5-dimethylpyrazole administration at dose 12 mg/kg body weight were observed (Locci and Bergamini, 1983). The antilipolytic agent 3, 5-dimethylpyrazole inhibits insulin release in response to both nutrient secretagogues and cyclic adenosine monophosphate (cAMP) agonists in isolated rat islets (Masiello et al., 2002).