www.eda-egypt.org • Codex : 101/1810 I.S.S.N 0070-9484 Oral Medicine, X-Ray, Oral Biology and Oral Pathology EGYPTIAN DENTAL JOURNAL Vol. 64, 3337: 3347, October, 2018 * Lecturers, Oral Pathology Department, Faculty of Oral and Dental Medicine, Cairo University ** Lecturers, Oral Surgery Department, Faculty of Oral Surgey, Misr University for Science and Technology. INTRODUCTION Cancer is group of cells developing from abnormal genetic makeup of a cell resulting in uncontrolled cell division. The cancer cells reveal aberrant cell growth, not responding to inhibitory growth signals with often production of metastasis throughout the body [1] One of the effective strategies to kill cancer cells is induction of apoptosis, a programmed cell death mechanism for removing unwanted cells in tissue [2] . This procedure is mainly associated with morphological change, heterochromatin INVESTIGATION THE CYTOTOXICITY AND APOPTOSIS OF ANNONACEAE NANOPARTICLES AND 5-FU ENCAPSULATED CHITOSAN NANOPARTICLES AGAINST TONGUE SQUAMOUS CELL CARCINOMA CELL LINES (SSC-25) Shaimaa Omar Zayed * , Reham S. Hamed * and Usama Abd El Rouf El Dakrory ** ABSTRACT Objective: cancer result from abnormal genetic makeup of a cell resulting in continuous cell division with often production of metastasis throughout the body. Apoptosis is very effective in killing cancer cells. We performed this study to investigate the effect of annonaceae and 5FU drug nanoparticles against scc-25 cell lines human tongue squamous cell carcinoma by studying their effect on cell cycle progression and apoptosis Methods: Cell Line cells were obtained from American Type Culture Collection , cells were cultured using DMEM (Invitrogen/Life Technologies). Annonaceae nanoparticles and 5FU drug were used. The effect of the tested materials were investigated by MTTassay, cell cycle analysis & fow cytometry. Results: the cancer cells treated by annonaceae nanoparticles revealed obvious cytotoxicity at iC50 7 µg/ml, in addition it stimulated the upregulation of annexin-v leading to apoptosis of cancer cells and growth arrest at G1 phase with prominent late apoptosis analyzed by cell cycle analysis & fow cytometry. Conclusion: Our fndings demonstrate that annonaceae nanoparticle might be capable of inducing cancer cell death against SCC-25 cell lines by cell cycle arrest and induction of apoptosis.