~ 1420 ~ Journal of Pharmacognosy and Phytochemistry 2019; 8(6): 1420-1425 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2019; 8(6): 1420-1425 Received: 01-09-2019 Accepted: 03-10-2019 Prabhat Gangwar Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur, Madhya Pradesh, India PC Shukla Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur, Madhya Pradesh, India Amita Tiwari Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur, Madhya Pradesh, India G Das Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur, Madhya Pradesh, India Shivangi Sharma Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur, Madhya Pradesh, India Shivangi Udainiya Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur, Madhya Pradesh, India Corresponding Author: Prabhat Gangwar Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur, Madhya Pradesh, India Haemato-biohemial alterations and comparative efficacy of different therapeutic regimen against trypanosomosis in buffaloes Prabhat Gangwar, PC Shukla, Amita Tiwari, G Das, Shivangi Sharma and Shivangi Udainiya Abstract The present study was conducted to know the haemato-biochemical alternations and to compare the therapeutic efficacy of different anti-trypanosomal drugs in buffaloes. Total of 118 buffaloes of either sex, age group and parity were screened for trypanosomosis. The haematological alternations in the infected buffaloes showed decreased pre treatment values of Hb, PCV, TEC which got increased on 3rd and 7 th day post treatment in all the groups. But Erythrocytic indices remained altered with no significance. The mean value of TLC (10 3 /μl) noted markedly decreased on post treatment in all the groups.The biochemical studies revealed no alternation in the mean values of Total protein in all the treated groups on post treatment than the pre treatment mean values 1 st day. On the contrary, marked increased mean values of glucose have been obtained on 3 rd and 7 th days post treatment in the T3 group. The AST values found under study were reported to be significantly reduced in all the groups post treatment, but maximum reduction was obtained in T3 groups on day 3 rd day 7 th . It was concluded that out of the drugs used isometamidium chloride was found to be most effective as evident by the maximum clinical recovery. Keywords: Trypanosomiasis, hemoprotozoan, buffaloes, hemato-biochemical Introduction Trypanosomosis is a haemoprotozoan disease entity caused by various members of Trypanosoma spp affecting different species of domestic and wild animals like horses, camel, cattle, buffaloes, deer, foxes, tiger and jackals with clinical signs as intermittent fever, anaemia, loss of weight, edema of dependent parts, nervous symptoms and abortion (Jaiswal et al., 2015) [17] . Bovine trypanosomosis (Surra) caused by Trypanosoma evansi is an important haemoprotozoan disease of buffalo which is characterized by high temperature, progressive anaemia and cutaneous eruptions. Infection is mechanically transmitted by blood-sucking insects of the genera Tabanus, Stomoxys etc but in India mostly by tabanid biting files (Vijay et al., 2002) [30] . It is a major killer disease of livestock that leads to major economic losses to the farmers in view of morbidity and mortility, decreased milk yield. Extensive use of trypanocides and by interference with vaccination programme of domestic animals in India. The parasite causes severe anemia, edema, immune suppression and various neurological signs by entering the nervous tissue resulting in death of affected animals. It is repetation in cattle and buffaloes act as carriers most frequently in some of the parts in india. Trypanosomosis directly affects the productivity of cattle by reducing birth rates, increasing abortion rates and increasing mortality rates (Mersha et al., 2013) [20] . In India, diminazene aceturate, Quinapyramine sulphate and chloride (Antrycide Prosalt) and Quinapyramine sulphate (Antrycide) are currently available drugs for treatment and prophylactic use against trypanosomosis in domestic animals (Ponnudurai et al., 2015) [26] . Material and methods Location and place of work The work was conducted in the Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur and nearby dairy farms in and around Jabalpur. Animals A total of 118 animals were screened on the basis of common clinical signs of trypanosomosis viz. Anorexia, temperature, edema of dependent parts, head pressing, excitement, circling, salivation, ocular discharge etc.