376 EFFECTIVENESS OF POMELO (Citrus maxima) AND LIME (Citrus aurantifolia) ORANGE PEEL EXTRACT AS Aedes aegypti BIOLARVASIDA Fajar Akbar *) , Fahrul Islam Department of Environmental Health, Mamuju Health Polytechnic *) E-mail: fajarpoltekkes@gmail.com ABSTRACT Dengue Hemorrhagic Fever in Indonesia has been a public health problem for the past 41 years. Since 1968 there has been an increase in the number of provinces and districts / cities endemic to DHF, from 2 provinces and 2 cities, to 32 (97%) and 382 (77%) districts / cities in 2009 (1). Orange plants with various varieties contain ingredients that can be used as natural insecticides in larvae. This study included the type of experiment with a completely randomized design that was testing the effectiveness of grapefruit peel extract (Citrus grandis) and lime (Citrus aurantifolia) as Aedes aegypti biolarvasida. This research will be conducted at the Mamuju Ministry of Health Polytechnic Building. The time of this study was in March - August 2018. The object of this study was orange peel extract which was exposed to instar III and IV Aedes aegypti larvae. The stages of research activities include making orange peel extract, preliminary test phase and research test phase. The results showed that the number of larval deaths exposed to lime peel extract (Citrus aurantifolia) with an average mortality of 97.3% was effective in killing Aedes aegypti larvae because more than 50% compared to the skin extract of grapefruit skin extract (Citrus grandis) with the average mortality is 1.3% so it is not effective in killing Aedes aegypti larvae because it is less than 50%. Keywords: extract, orange, peel, aedes aegypti INTRODUCTION Dengue Hemorrhagic Fever in Indonesia has been a public health problem for the past 41 years. Since 1968 there has been an increase in the number of provinces and districts / cities that are endemic for DHF, from 2 provinces and 2 cities, to 32 (97%) and 382 (77%) districts / cities in 2009. The number of patients and the area spread increased due to high population mobility, development of urban areas, climate change, changes in population density and distribution and other epidemiological factors that still need further research (1). The case of 2011 dengue hemorrhagic fever in Mamuju Regency recorded 173 people suffering from dengue and the death was only 1 person. In 2012 the number of dengue cases was recorded by 100 people and 1 died. The highest incidence of cases in the Binanga Health Center area in five kelurahan in a row from 2013 there were 85 cases died 1 person and 2014 as many as 48 people none died. And in 2017 the number of dengue cases in Mamuju Regency was 20 cases of DHF and 1 person died, of which 15 cases of dengue occurred in the work area of the Binanga Health Center (2). The use of repetitive insecticides can increase the risk of contamination of pesticide residues in water, especially drinking water. Temephos cannot be used orally, so its presence is not expected to be in drinking water. Another important thing to note is the high cost of using chemical pesticides and the emergence of resistance from various species of mosquitoes which become vectors of disease. With the above problems, it is necessary to do research on alternative natural materials to overcome the spread of Aedes aegypti mosquitoes. The use of natural larvacides has several advantages, including rapid degradation by sunlight, air, moisture and other natural components, thereby reducing the risk of soil and water pollution. Natural larvasida has low toxicity in mammals, so natural larvicidal use allows it to be applied to human life. The choice of material to be used as larvacide must be safe against humans or other organisms, besides that the material is easily obtained and is expected to have a positive impact on human health (3). One of the development of alternative insecticides is by killing mosquitoes, especially in the larval stage using natural larvicides. With this effort, it is expected that the development of the life cycle will be hampered or disconnected because mosquitoes cannot develop into adults. The results showed that larvacides derived from plant extracts are safe for the environment, can be degraded, and are specific to the target (4). Orange plants with various varieties contain ingredients that can be used as natural insecticides in larvae. Based on phytochemical screening performed (5) showed that orange peel contains tannins, saponins, phytate oxalates, flavonoids, and limonoid. Limonoid has the ability to inhibit