Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1, April 2015 55 EFEKTIVITAS VAKSINASI Crude DAN Soluble Protein SPORA Myxobolus koi TERHADAP TINGKAT KERUSAKAN USUS IKAN KOI (Cyprinus carpio Koi) THE EFFECTIVETY Crude and Soluble Protein of Myxobolus koi SPORE AGAINTS INTESTINE DIFFERENT DEGREES IN KOI (Cyprinus carpio koi). Rachma Woro Anggarani, Gunanti Mahasri dan Lucia Tri Suwanti Fakultas Perikanan dan Kelautan Universitas Airlangga Kampus C Mulyorejo - Surabaya, 60115 Telp. 031-5911451 Abstract Myxobolus koi is one species of Myxobolus sp that can cause parasitic diseases in fish called Myxobolusis. Based on the Decree of the Minister of Marine and Fisheries No: KEP.03/MEN/2010 that Myxobolus koi in the list of Fish Quarantine Pests group I. Myxosporean diseases are most numerous in the water can cause Proliferative Kidney Disease (PKD) and Whirling Disease (WD). The aim of this research is to finding, analyzing and determining the protein of spores Myxobolus koi that can effectively reduce the level of damage to the intestinal organs as well as for the prevention myxobolusis on Koi’s. Then for finding, analyzing and determining the protein of spores Myxobolus koi do isolation of spore proteins. The study consisted of three phases examination to preparation and identification of spores, isolation and analyze of crude and soluble protein spores for obtain dose and molecular weight each protein and histopathological test. This research uses descriptive method. The data presented may be narratives, images, tables or charts for each group. Intestinal histopathology test results of research carried scoring Koi’s were analyzed using the Kruskal -Wallis. The results showed a profile crude protein and soluble proteins from spores Myxobolus koi showed that the molecular weight of crude protein Myxobolus koi in this study was 150 kDa and 72 kDa and for soluble protein was 73 kDa. Results scoring the degree of infection caused by exposure to Myxobolus koi then statistically processed with an average yield of scoring in a sequence of 0; 1.6; 0.64 and 0.32. Statistical analysis showed no significant difference in the treatment of K + with crude protein, and K + with soluble proteins. Statistical analysis showed that there were significant differences in treatment with K+ and K-, K- with soluble protein and crude protein and soluble protein. Histopathological changes in the intestine in the form of inflammatory cell infiltration, necrosis and haemorage Keywords : Myxobolus, crude protein, soluble protein, histopathology, intestines, koi fish Pendahuluan Myxobolus koi merupakan salah satu spesies dari Myxobolus sp yang dapat menyebabkan penyakit parasiter pada ikan yang disebut Myxobolusis. Pada umumnya organisme penyebab penyakit ini bersifat sistemik serta dikenali dari morfologi spora, jumlah dan lokasi filamen polar (Mahasri, 2004). Mahasri dan Kismiyati (2011) menjelaskan bahwa Myxobolus koi membentuk nodul pada insang yang berisi ribuan spora sehingga dapat menghalangi proses penyerapan oksigen serta sering menyebabkan operculum selalu terbuka. Jika nodul ini pecah, maka spora menyebar ke perairan sehingga tertelan oleh ikan akibat ukuran spora yang relatif kecil. Spora yang tertelan oleh ikan akan masuk kedalam usus kemudian melepaskan filamen polar untuk menancap pada dinding usus serta mengeluarkan sporoplasma untuk melakukan penetrasi pada mukosa usus dan mengikuti aliran darah menuju organ target yakni insang. Usus adalah organ utama pencernaan makanan dan penyerapan nutrisi. Takashima and Hibiya (1995) menjelaskan bahwa struktur histologi dari dinding saluran pencernaan ikan terdiri dari empat lapisan dasar yakni mukosa, submukosa, muskularis dan serosa. Menurut Deloshoub et al (2010) lapisan mukosa usus memiliki banyak vili sepanjang anterior hingga posterior usus dan pada bagian mukosa inilah banyak ditemukan infeksi parasit. Upaya pengobatan dengan menggunakan perendaman air laut maupun penggunaan bahan kimia seperti formalin, methylen blue, pottasium permanganate, glacial acetic acid atau phenol tidak efektif untuk