International http Anticariogenic poten Alka Choudhary 1 , Umesh Bih *Corresponding author: Uttam Chand Banerjee 1 Department of Natural Products, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062, Punjab, India. 2 Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062, Punjab, India. 3 Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong, 793002, Meghalaya, India. A St fu Ba ex to ep (M an as Th na Ke Ti Introduction Dental caries, a multifactorial complication is interaction between dietary factors, oral environm Of these three factors, bacteria, Streptococci an the major players in causing incidence and pre caries among the people of different age grou attach to the teeth enamel and form a glycocaly sticky glucan by action of bacterial enzymes, gluc on sucrose resulting in formation of plaque. Bact inside plaque. This low pH demineralises the too bacteria are very efficient in trapping their sub carbohydrates from the diet or its left overs in considerable period of time. Several anti-bacteria as well as natural products, are reported to com caused by cariogenic bacteria to teeth enamel [1 is considered as a gold standard in treatment however its long term use unveils unexpected teeth staining, and taste change, desquamations the oral mucosa restricting its routine use [3]. Th continuous research and discovery of newer antic DOI:10.5138/09750185.1939 This article is distributed under the terms o and redistribution provided that the origina l Journal of Phytomedicine 9 (2017) p://www.arjournals.org/index.php/ijpm/index Original Research Article ntial of Potentilla fulgens extract and constituents hade 2 , Amit Kumar Mittal 2 , Anupam Chatterjee 3 , Utta Inder Pal Singh 1, Abstract Streptococci and Lactobacilli are the most common bacteria ca uglens, native of north-east India is used by tribal people to c ased on its ethomedical use, its anticariogenic potential was ev xtract was found to have good anticariogenic potential. Epigallo o the most effective inhibitor against the tested bacterial s picatechin (8) also demonstrated comparatively better activity th MIC of 1.56, 3.12 and 3.12 μg/mL against Streptococcus muta nd Lactobacillus rhamnosus, respectively). Time-kill studies an ssays showed molecule 8 to be comparable to compound 4 in he results suggest that these compounds and Potentilla ext atural antibacterial agents in oral health care products. eywords: Potentilla fuglens, Streptococcus mutans, Anticari ime-kill study, Biofilm inhibition s an outcome of ment and bacteria. nd Lactobacilli are evalence of dental ups. The bacteria yx by synthesizing cosyl transferases teria produce acid oth enamel. These bstrates, generally the mouth over a al agents, synthetic mbat the damage 1-2]. Chlorhexidine of dental caries; d side-effects, like s and soreness in here is a need for cariogenic agents. Plant polyphenols have attracted several years as potential anticari known to prevent oral diseases b enzymes, inducing apoptosis monocytes/macrophages to pr myeloperoxidase-dependent iodi inactivating bacterial toxins [4-6]. including Asteraceae, Fabaceae, R Geraniaceae, Onagraceae, Poly Verbenaceae are reported to poss these families, members of the families are a rich source of p antimicrobial activity [7-9]. Genus Potentilla, a member of Ros polyphenols, reported to contain a polyphenols including, hydrolysable Potentilla recta extracts and its va partitioning with solvents of diff ethanol, diethyl ether, ethyl a demonstrated in vitro inhibitor Streptococcus spp. strains. These e of oral Streptococci and had inhi of the Creative Commons Attribution License, which p al author and source are credited. ) 83-91 d its chemical am Chand Banerjee 2* , ausing dental caries. Potentilla cure teeth and gum problems. valuated. The crude methanol ocatechin gallate (4) was found strains. Epiafzelechin (4β 8) han other dimeric compounds utans, Lactobacillus acidophilus nd biofilm formation inhibition n terms of antibacterial action. tracts could be employed as iogenic, Dimeric flavan-3-ols, d substantial attention in past iogenic agents. Polyphenols are by inhibiting bacterial replication in tumour cells, stimulating roduce cytokines, triggering ination of neutrophils and . Plants from different families, Rosaceae, Poaceae, Lythraceae, ygonaceae, Primulaceae, and sess bactericidal action. Among e Geraniaceae and Rosaceae polyphenolic compounds with saceae family, is a rich source of a variety of structurally diverse e tannins and flavanoids [10-12]. arious subfractions obtained by ferent polarity (aqueous, 50% acetate and n-butanol) have ry effects against cariogenic extracts also inhibited the growth ibitory effect on water-insoluble ISSN: 0975-0185 permits unrestricted use