Received: 30 September 2017, Accepted: 18 November 2017 Subject Editor: Mehran Ghazavi Journal of Entomological Society of Iran 2018, 37(4), supplementary, 507514 ﻧﺎﻣﻪ اﻧﺠﻤﻦ ﺣﺸﺮه ﺷﻨﺎﺳﯽ اﯾﺮان514 - 507 ﺿﻤﯿﻤﻪ ,) 4 ( 37 , 1396 Doi: 10.22117/jesi.2018.115654.1139 Special Issue: Proceedings of the 2 nd Iranian International Congress of Entomology The bacterial infection of German cockroach (Blatella germanica) in hospitals of western Iran Amir Keyhani 1 , Amir Hossein Zahirnia 2 , Jalil Nejati ,3 & 4 , Ehssan Mozaffari 4 , Abedin Saghafipour 5 & Alireza Omidi Oskouei 5 1-Department of Medical Parasitology, Kerman University of Medical Sciences, Kerman, Iran, 2- Department of Medical Entomology & Vector control, School of Medicine, Hamadan University of Medical Sciences, Hamedan, Iran, 3-Health Promotion Research Center, Zahedan University of Medical Sciences, Zahedan, Iran, 4-Department of Medical Entomology & Vector control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran & 5-Department of Public Health, Faculty of Health, Qom university of Medical Sciences, Qom, Iran *Corresponding author, E-mail: jalilnejati@yahoo.com Abstract Cockroaches are considered a serious health problem in some medical centers. They can move freely in hospital wards and transmit pathogenic organisms. This study was carried out in order to detect and identify bacterial infection on outer surface and in digestive organs of Blatella germanica as a dominant species of cockroach in hospitals. In this cross-sectional study 222 B. germanica were collected from 14 different wards and units of 5 hospitals in the city of Hamedan in western Iran. Most of them were collected from dining room (34.2%) followed by urology ward (13.5%). After applying anesthesia and washing in 2cc physiology serum, the culture was prepared from this suspension for identification of bacterial infection on outer surface. Next, the outer surface of each cockroach was sterilized and their guts were isolated from mouth to anus. Bacteria extraction and culture were done accordingly. A total of 12 bacteria species were identified from outer surface and digestive system of cockroaches. On outer surface, Escherichia coli was the most dominant species (40%) followed by Staphylococcus epidermidis (20%). In digestive system E. coli had also the highest percentage (45%) followed by Entrobacter aeroginosa (20%). This study can show the importance of cockroaches as potential vectors of medically important microorganisms such as pathogenic bacteria in hospitals. Control programs for removing cockroaches, especially in the hospitals are recommended. Key word: Blatella germanica, Bacterial infection, Hospital, Iran آﻟﻮدﮔﯽ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﺳﻮﺳﺮي آﻟﻤﺎﻧﯽ(Blatella germanica) در ﺑﯿﻤﺎرﺳﺘﺎن ﻫﺎ، ﻏﺮب اﯾﺮان اﻣﯿﺮ ﮐﯿﻬﺎﻧﯽ 1 ، اﻣﯿﺮﺣﺴﯿﻦ ﻇﻬﯿﺮﻧﯿﺎ 2 ، ﺟﻠﯿﻞ ﻧﺠﺎﺗﯽ 3 و4 و* ، اﺣﺴﺎن ﻣﻈﻔﺮي 4 ، ﻋﺎﺑﺪﯾﻦ ﺛﻘﻔﯽ ﭘﻮر 5 و ﻋﻠﯿﺮﺿﺎ اﻣﯿﺪي اﺳﮑﻮﯾﯽ 5 1 - ﮔﺮوه اﻧﮕﻞ ﺷﻨﺎﺳﯽ و ﻗﺎرچ ﺷﻨﺎﺳﯽ ﭘﺰﺷﮑﯽ، داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ، داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﮐﺮﻣﺎن، 2 - ﮔﺮوه ﺣﺸﺮه ﺷﻨﺎﺳﯽ ﭘﺰﺷﮑﯽ و ﻣﺒﺎرزه ﺑﺎ ﻧﺎﻗﻠﯿﻦ، داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ، داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﻫﻤﺪان، 3 - ﻣﺮﮐﺰ ﺗﺤﻘﯿﻘﺎت ارﺗﻘﺎء ﺳﻼﻣﺖ، داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ زاﻫﺪان، 4 - ﮔﺮوه ﺣﺸﺮه ﺷﻨﺎﺳﯽ ﭘﺰﺷﮑﯽ و ﻣﺒﺎرزه ﺑﺎ ﻧﺎﻗﻠﯿﻦ، داﻧﺸﮑﺪه ﺑﻬﺪاﺷﺖ، داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺗﻬﺮان و5 - ﮔﺮوه ﺑﻬﺪاﺷﺖ ﻋﻤﻮﻣﯽ، داﻧﺸﮑﺪه ﺑﻬﺪاﺷﺖ، داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﻗﻢ * ﻣﺴﺌﻮل ﻣﮑﺎﺗﺒﺎت، ﭘﺴﺖ اﻟﮑﺘﺮوﻧﯿﮑﯽ:jalilnejati@yahoo.com ﭼﮑﯿﺪه ﺳﻮﺳﺮي ﻫﺎ ﺑﻪ ﻋﻨﻮان ﯾﮏ ﻣﺸﮑﻞ ﺑﻬﺪاﺷﺘﯽ ﺟﺪي در ﻣﺮاﮐﺰ درﻣﺎﻧﯽ ﻣﺤﺴﻮب ﻣﯽ ﺷﻮﻧﺪ. آﻧﻬﺎ ﻣﯽ ﺗﻮاﻧﻨﺪ آزاداﻧﻪ در ﺑﺨﺶ ﻫﺎي ﻣﺨﺘﻠﻒ ﺑﯿﻤﺎرﺳﺘﺎﻧﯽ ﺣﺮﮐﺖ ﮐﺮده و ﻋﻮاﻣﻞ ﺑﯿﻤﺎرﯾﺰا را ﺟﺎﺑﺠﺎ ﻧﻤﺎﯾﻨﺪ. اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺎ ﻫﺪف ﺑﺮرﺳﯽ آﻟﻮدﮔﯽ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﺳﻄﺢ ﺧﺎرج ﺑﺪن و دﺳﺘﮕﺎه ﮔﻮارش ﺳﻮﺳﺮي آﻟﻤﺎﻧﯽ(B. germanica) ، ﺑ ﻪ ﻋﻨﻮان ﯾﮏ ﮔﻮﻧﻪ ﻏﺎﻟﺐ ﺳﻮﺳﺮي در ﺑﯿﻤﺎرﺳﺘﺎن ﻫﺎ، اﻧﺠﺎم ﮔﺮﻓﺖ. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﻣﻘﻄﻌﯽ ﺗﻌﺪاد222 ﻋﺪد ﺳﻮﺳﺮي آﻟﻤﺎﻧﯽ از14 ﺑﺨﺶ در5 ﺑﯿﻤﺎرﺳﺘﺎن ﺷﻬﺮ ﻫﻤﺪان ﺻﯿﺪ ﺷﺪﻧﺪ. ﺳﻠﻒ ﺳﺮوﯾﺲ و ﺑﺨﺶ اروﻟﻮژي ﺑ ﻪ ﺗﺮﺗﯿﺐ ﺑﯿﺸﺘﺮﯾﻦ درﺻﺪ ﺻﯿﺪ را داﺷﺘﻨﺪ. ﺑﻪ ﻣﻨﻈﻮر ﺗﻌﯿﯿﻦ آﻟﻮدﮔﯽ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﺧﺎرﺟﯽ، ﺳﻮﺳﺮي ﻫﺎ ﺑﻌﺪ از