Nanomed. J. 7(3): 243-250, Summer2020 RESEARCH PAPER Preparation and study of the inhibitory efect of nano-niosomes containing essential oil from artemisia absinthium on amyloid fbril formation Mojtaba Ansari * , Hossein Eslami Department of Biomedical Engineering, Meybod University, Meybod, Iran * Corresponding Author Email: ansari@meybod.ac.ir Note. Tis manuscript was submitted on February 10, 2020; approved on April 15, 2020 How to cite this article Ansari M, Eslami H. Preparation and study of the inhibitory efect of nano-niosomes containing essential oil from artemisia absinthium on amyloid fbril formation. Nanomed J. 2020; 7(3): 243-250. DOI: 10.22038/nmj.2020.07.0009 ABSTRACT Objective(s): Artemisia absinthium is an aromatic, perennial small shrub that shows multiple medical benefts, including anticancerous, neuroprotective, antifungal, hepatoprotective, antidepressant and antioxidant properties. One of the efective approaches to treat Alzheimer’s disease is targeting amyloid aggregation by antiamyloid drugs. In the current research study, an excellent grouping of niosomal, lipid nano-carriers drugs containing artemisia absinthium is advanced and characterized to inhibit amyloid aggregation. Materials and Methods: Niosomal vesicles were made employing phosphatidylcholine, span 60, cholesterol and DSPE-PEG2000 by the thin-flm method. Ten artemisia absinthium was loaded into the niosomes. Teir physico-chemical attributes were analyzed utilizing Zeta-Sizer, FTIR, and SEM, and the amount of drug release was measured at 37° C. Finally, the inhibitory efect of artemisia absinthium that loaded niosomal vesicles on the aggregation of amyloid-β peptides was investigated using Tioflavin T fluorescence measurements and atomic force microscopy. Results: Niosomes containing artemisia absinthium have a size of 174±2.56nm, the encapsulation efciency of 66.73%, zeta potential of -26.5±1/42 mV and polydispersity index (PDI) of 0.373±0/02. Te release of the drug is controlled in this nano-carrier and FTIR and SEM investigations showed that the drug and nano-carrier did not interact and their particles had a spherical structure. In the end, the inhibitory efect of artemisia absinthium that loaded niosomal vesicles on the aggregation of amyloid-β peptides was examined and confrmed through Tioflavin T fluorescence measurements and atomic force microscopy. Conclusion: Meanwhile, the fndings of the current study, confrm the appropriate physicochemical features of the system, a slow-release system, show that this nano-carrier inhibits amyloid aggregation, thus, the nano-niosomes containing essential oil from artemisia absinthium has the capability to preclude amyloid development. Keywords: Alzheimer’s disease, Amyloid-β aggregation, Niosome, Artemisia absinthium, Drug delivery INTRODUCTION Accumulaton of proteins and peptdes into toxic amyloid formatons is a crucial biological occurrence that gives rise to the onset of diferent destructve pathologies, comprising plenty of neurodegeneratve diseases [1]. More than 50 amyloidogenic proteins and peptdes by Amyloid aggregate formaton have been realized to induce a couple of a diseases, including Parkinson’s disease, Huntngton’s disease, and Alzheimer’s disease [1-5]. Most proteins are chiefy alpha-helix. Amyloid fbers, by contrast, characteristcally undergo the transiton from the typically soluble form into amyloid fbrils classifed mostly into cross- beta-sheet, which pile up in the extracellular space of numerous tssues [6]. Amyloid-beta is a consttuent of cerebrospinal fuid and plasma of robust individuals in the soluble form and is secreted by normal cells [7]. In Alzheimer’s disease, this Aβ (Amyloid-beta (1-42)) peptdes can self-assemble to consttute neurological toxic aggregates with numerous morphologies, such as soluble oligomers and insoluble protofbrils and fibrils [8,9]. Consequently, it is signi fcant to preclude amyloid aggregaton-associated pathologies, and one