~ 145 ~
The Pharma Innovation Journal 2021; SP-10(3): 145-149
ISSN (E): 2277- 7695
ISSN (P): 2349-8242
NAAS Rating: 5.23
TPI 2021; SP-10(3): 145-149
© 2021 TPI
www.thepharmajournal.com
Received: 06-01-2021
Accepted: 09-02-2021
Dharmendra Singh
Faculty of Veterinary and Animal
Sciences, WBUAFS Belgachia
Kolkata, West Bengal, India
Ajay Prakash
Department of Anatomy, College of
Veterinary Science & Animal
Husbandry U.P. Pandit Deen Dayal
Upadhyaaya Pashu Chikitsa Vigyan
Vishwavidyalay Mathura, Uttar
Pradesh, India
MM Farooqui
Department of Anatomy, College of
Veterinary Science & Animal
Husbandry U.P. Pandit Deen Dayal
Upadhyaaya Pashu Chikitsa Vigyan
Vishwavidyalay Mathura, Uttar
Pradesh, India
Sri Prakash Singh
Department of Anatomy, College of
Veterinary Science & Animal
Husbandry U.P. Pandit Deen Dayal
Upadhyaaya Pashu Chikitsa Vigyan
Vishwavidyalay Mathura, Uttar
Pradesh, India
Avnish Kumar Gautam
Faculty of Veterinary and Animal
Sciences, WBUAFS Belgachia
Kolkata, West Bengal, India
Ajeet Kumar Jha
Faculty of Veterinary and Animal
Sciences, WBUAFS Belgachia
Kolkata, West Bengal, India
Keshav Kumar
Faculty of Veterinary and Animal
Sciences, WBUAFS Belgachia
Kolkata, West Bengal, India
Corresponding Author:
Avnish Kumar Gautam
Faculty of Veterinary and Animal
Sciences, WBUAFS Belgachia
Kolkata, West Bengal, India
Histomorphogenesis of exocrine part of pancreas in
prenatal goat (Capra hircus)
Dharmendra Singh, Ajay Prakash, MM Farooqui, Sri Prakash Singh,
Avnish Kumar Gautam, Ajeet Kumar Jha and Keshav Kumar
Abstract
The stained foetal serial sections of early prenatal stage showed that the primordium of foetal goat
pancreas was present in close vicinity of developing duodenum. At 69 day old goat foetus the pancreatic
parenchyma was formed by tubules of different length and diameter, tubular buds, blood capillaries and
islets of Langerhans. The beginning of the process of acini formation in the foetal goat pancreas was first
observed from 76 days of gestation. Cells of several buds became loosely arranged and their peripheral
cells began to arrange in the form of either a complete or a part of circle to form a central lumen. From
115 days onwards the cytoplasm of few acinar cells around the nucleus had a little bluish tinge and
centroacinar cells were seen in few acini. From 132 days to full term foetus the pancreas was chiefly
formed by developed acini, however at few places some immature acini were also found.
Keywords: Pancreas, histogenesis, exocrine part, prenatal goat
Introduction
The pancreas is an organ containing two distinct population of cells, the exocrine cells that
secrete enzyme into the digestive tract and endocrine cells that secrets hormones into the
bloodstream (J.M.W. Slack, 1995)
[10]
. The exocrine parts made up of acini or alveoli. Each
acinus has a single layer of acinar cells with a lumen in the centre. The acinar cells contain
zymogen granules, which possess digestive enzyme i.e. pancreatic juice. These enzymes have
greatest role in digestion of proteins, lipids and carbohydrate. So alteration and impairment in
the structure of foetal exocrine parts cause profound effects on performance of goat.
Publication devoted to the histogenesis of these part of foetal goat pancreas is scanty.
Therefore, present study was undertaken to see the origin and development of these parts of
pancreas during different stage of gestation.
Material and Methods
The present study was conducted on the pancreas of 24 goat foeti of either sex ranging from 42
days to 147 days of gestation. In current research, ethical approval is exempted from the
university. The samples were taken from the aborted foeti which has collected from the clinics
of college of Veterinary Science & Animal Husbandry, Mathura and local Veterinary hospital
of Mathura, Uttar Pradesh, India. The approximate age of foeti were estimated by using the
formula derived by Singh et al. (1979)
[9]
. in goat. The foeti were divided into early prenatal (0
to 50 days gestation), mid prenatal (51 to 100 days gestation) and late prenatal (101 to 147
days gestation) periods. As the separation of the pancreas from the adjacent organ was not
possible before 50 days gestation, therefore from 42 and 44 days old foeti a mass of the tissue
having group of the organ was collected and was fixed in neutral buffered formalin. Tissues
were processed by standard protocol of tissue processing and five to six micron thick paraffin
sections were obtained. The sections were stained with haematoxylin and eosin for general
histoarchitecture, Wilder’s reticular stain for reticular fibres, Verhoeff’s method for elastic
fibres and Masson’s trichome stain for collagen fibres (Luna, 1968)
[7]
. The micrometric
observations were conducted with the help of computerized Motic Image Pluse 2.0 software.
For each micrometric parameter 10 readings were taken at different places in the same slide.
Results
Early Prenatal Stage
The stained serial sections of 42 days old foetus showed that the primordium of foetal goat