Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Tue, 06 Nov 2018 18:39:08 Aliidiomarina iranensis sp. nov., a haloalkaliphilic bacterium from a coastal-marine wetland Mohammad Ali Amoozegar, 1 Azadeh Shahinpei, 2 Seyed Abolhassan Shahzadeh Fazeli, 2 Peter Schumann, 3 Cathrin Spro ¨er 3 and Antonio Ventosa 4 Correspondence Mohammad Ali Amoozegar amoozegar@ut.ac.ir 1 Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran 2 Microorganisms Bank, Iranian Biological Resource Centre (IBRC), Academic Center for Education, Culture and Research, Tehran, Iran 3 Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany 4 Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain A novel Gram-stain-negative, straight rod-shaped, non-pigmented, slightly halophilic and alkaliphilic bacterium, designated strain GBPy7 T , was isolated from a sample of the coastal- marine wetland Gomishan in Iran. Cells of strain GBPy7 T were motile. Growth occurred on media with 1–15 % (w/v) NaCl (optimum 3 %), at pH 7–10 (optimum pH 8.5) and at 4–45 8C (optimum 37 8C). Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that strain GBPy7 T belonged to the family Idiomarinaceae. Its closest relatives were Aliidiomarina shirensis AIS T (98.1 % 16S rRNA gene sequence similarity) and other Aliidiomarina species (95.9–94.2 %), together with Idiomarina seosinensis CL-SP19 T (94.3 %) and Idiomarina fontislapidosi F23 T (94.3 %). The major cellular fatty acids of the isolate were iso-C 15 : 0 , iso-C 17 : 0 , iso-C 17 : 1 v9c and C 18 : 1 v7c and its polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown aminophospholipid. Cells of strain GBPy7 T contained ubiquinone Q-8. The G+C content of the genomic DNA of this strain was 51.6 mol%. The level of DNA–DNA relatedness between strain GBPy7 T and A. shirensis IBRC-M 10414 T was 21 %. The physiological, biochemical, genotypic and phylogenetic differences between strain GBPy7 T and other previously described taxa indicate that the strain represents a novel species of the genus Aliidiomarina within the family Idiomarinaceae, for which the name Aliidiomarina iranensis sp. nov. is proposed. The type strain is GBPy7 T (5IBRC-M 10763 T 5CECT 8339 T ). The family Idiomarinaceae, within the class Gammaproteo- bacteria, was proposed by Ivanova et al. (2004). Members of this family are aerobic or facultatively anaerobic chemo-organotrophs, use carbohydrates poorly as sole carbon and energy sources and require sodium ions for growth. Members of the family have been isolated from open and deep-sea waters. The fatty acids of this family are dominated by iso-C 15 : 0 and iso-C 17 : 0 (Ivanova et al., 2004; Jean et al., 2006) and the major isoprenoid quinone is ubiquinone 8 (Q-8) (Taborda et al., 2009). The family currently comprises two genera: Idiomarina (Ivanova et al., 2000) and Aliidiomarina (Huang et al., 2012), as species of the genus Pseudidiomarina (Jean et al., 2006) were reassigned to the genus Idiomarina (Taborda et al., 2009). The genera Idiomarina and Aliidio- marina cannot be differentiated on the basis of phenotypic characteristics, and signature nucleotides in the 16S rRNA gene sequences have been defined for the family Idiomari- naceae (Taborda et al., 2009). The type species of the genus Aliidiomarina, Aliidiomarina taiwanensis, was isolated from a seawater sample collected in the shallow coastal region of Bitou Harbour, Taiwan (Huang et al., 2012). To date, four other species have been described in this genus, Aliidioma- rina haloalkalitolerans (Srinivas et al., 2012), ‘Aliidiomarina sanyensis’ (Wang et al., 2013), Idiomarina maris which was reclassified within the genus Aliidiomarina as Aliidiomarina The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain GBPy7 T is KF724186. International Journal of Systematic and Evolutionary Microbiology (2016), 66, 2099–2105 DOI 10.1099/ijsem.0.000996 000996 G 2016 IUMS Printed in Great Britain 2099