AACL Bioflux, 2016, Volume 9, Issue 5. http://www.bioflux.com.ro/aacl 944 Growth performance and survival rate of climbing perch ( Anabas testudineus ) fed Daphnia sp. enriched with manure, coconut dregs flour and soybean meal Dedi F. Putra, Muhammad Fanni, Zainal A. Muchlisin, Abdullah A. Muhammadar Department of Integrated Coastal Resource Management, Graduate School of Syiah Kuala University, Banda Aceh 23111, Indonesia. Corresponding author: D. F. Putra, dfputra@unsyiah.ac.id Abstract . The objective of the present study was to determine the best material for enhancing nutritional value of Daphnia fed to climbing perch ( Anabas testudineus) larvae. The study was conducted at Laboratory of Marine and Fishery Faculty, Syiah Kuala University, Indonesia in July 2015. The completely randomized design was used, this study was conducted with three enhancer materials; chicken manure, coconut dregs and soybean meals. The Annova test showed that the treatment gave a significant effect on specific growth rate, weight and length gains (p < 0.05), but did not give a significant effect on survival rate of climbing perch larvae (p > 0.05). The highest growth performance was found at fish fed with Daphnia enhanced by soybean meals. Hence, it is concluded that the best enrichment material of Daphnia for climbing perch larvae is soybean meal. Key Words: Kruep, betok, specific growth rate, weight gain, Daphnia. Introduction. Climbing perch ( Anabas testudineus) or locally known as kruep fish are living in freshwater, they can live in waters with low oxygen levels because they have an additional respiratory organ called labyrinth (Hughes et al 1986). This species commonly occurres in Aceh waters, Indonesia (Muchlisin & Siti-Azizah 2009; Muchlisin et al 2015). The climbing perch has thick flesh, delicate and tastes savory so favored by the local people, and therefore this fish has potency as a fish target for aquaculture (Muchlisin 2013). Presently, the kruep fish supply are mostly from wild, this is because culture system has not well developed. Intensive fishing on climbing perch gave a negative impact in decreasing of wild population of this species (Personal communication with fisheries local authority). Therefore, the catching climbing perch from wild population has to be stopped and the fisher has to switch their business to aquaculture, and therefore the hatchery technology of climbing perch has to be initiated to support the fish farming of this favorite fish in the future. Larval rearing is an important aspect in relation to producing the high quality and quantity of fish larvae. Daphnia is one of common live feeds for fish larvae. This live feed contains 20- 27% lipid and up to 70% protein (Pangkey 2009). The nutrient content of Daphnia is possible to be improved by feeding it the high nutritional materials. This technique is commonly known as enrichment method with the objective to increase the nutritious value of live feed and would give a positive effect on growth performance and survival of the fish larvae (Mufidah et al 2009). Several materials have been used as enrichment materials for Daphnia such as canola oil (Fereidouni et al 2013), n-3 HUFA (Gholomi 2010), potassium (Civitello et al 2014). Other promising materials are soybean meal and coconut dregs meal. The coconut dreg has 14.54% protein, while the soybean meal has approximately 53.44% protein (Aprilian 2014). These materials have not been used as enrichment in daphnia for climbing perch larvae live feed. Hence, the objective of the