Research Article Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine Ali Taghipour , 1 Sanaz Tavakoli , 2 Mohamad Sabaghan , 3 Masoud Foroutan , 4 Hamidreza Majidiani , 5 Shahrzad Soltani , 4 Milad Badri , 6 Ali Dalir Ghaffari , 1 and Sheyda Soltani 4 1 Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran 2 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 3 Behbahan Faculty of Medical Sciences, Behbahan, Iran 4 USERN Office, Abadan University of Medical Sciences, Abadan, Iran 5 Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran 6 Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Correspondence should be addressed to Mohamad Sabaghan; sabaghan.m@ajums.ac.ir and Masoud Foroutan; masoud_foroutan_rad@yahoo.com Received 10 April 2021; Revised 10 June 2021; Accepted 22 June 2021; Published 12 July 2021 Academic Editor: José F. Silveira Copyright © 2021 Ali Taghipour et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Apicomplexan parasites, including Toxoplasma gondii (T. gondii), express different types of calcium-dependent protein kinases (CDPKs), which perform a variety of functions, including attacking and exiting the host cells. In the current bioinformatics study, we have used several web servers to predict the basic features and specifications of the CDPK7 protein. The findings showed that CDPK7 protein has 2133 amino acid residues with an average molecular weight (MW) of 219085.79 D. The aliphatic index with 68.78 and grand average of hydropathicity (GRAVY) with -0.331 score were estimated. The outcomes of current research showed that the CDPK7 protein included 502 alpha-helix, 1311 random coils, and 320 extended strands with GOR4 method. Considering the Ramachandran plot, the favored region contains more than 92% of the amino acid residues. In addition, evaluation of antigenicity and allergenicity showed that CDPK7 protein has immunogenic and nonallergenic nature. The present research provides key data for more animal-model study on the CDPK7 protein to design an efficient vaccine against toxoplasmosis in the future. 1. Introduction Toxoplasma gondii is a prevalent intracellular protozoan, which can infect a broad spectrum of mammals (i.e., human) and birds [1, 2]. Oocysts are the potential infective form in the life cycle of the parasite. Feline species as the only definitive hosts can contaminate the environment by shed- ding unsporulated oocysts through feces [3]. T. gondii is transferred by water/vegetables contaminated via mature oocysts and consumption of raw or semicooked meat from infected animals, vertical transmission from infected preg- nant mothers to neonates, and blood transfusion [4–7]. Approximately one-third of human society has been exposed to T. gondii, worldwide [5, 8, 9]. Often T. gondii infection among immunocompetent people is asymptomatic or dem- onstrates mild symptoms, whereas in immunocompromised patients, it can cause a various range of clinical symptoms [6, 9, 10]. Toxoplasmosis in immunocompromised subjects can cause repeated attacks in the brain and manifests as encephalitis [11]. Moreover, toxoplasmosis in pregnant women can cause blindness, microcephaly, and mental retar- dation in the infant [6, 12]. Different factors, such as host’s Hindawi Journal of Parasitology Research Volume 2021, Article ID 9974509, 20 pages https://doi.org/10.1155/2021/9974509