Assessment of DNA-binding affinity of cholinesterase reactivators and electrophoretic determination of their effect on topoisomerase I and II activity Janockova J. a,b , Zilecka E. a , Kasparkova J. c , Brabec V. c , Soukup O. d , Kuca K. b , Kozurkova M. a,b a Institute of Chemistry, Department of Biochemistry, Faculty of Science, P. J. Safarik University, Moyzesova 11, 040 01 Kosice, Slovak Republic b Biomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, 500 05 Hradec Kralove, Czech Republic c Department of Biophysics, Faculty of Science, Palacky University, Slechtitelu 27, 783 71 Olomouc, Czech Republic d Department of Toxicology and Military Pharmacy, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic 450 400 350 300 250 1 0,8 0,6 0,4 0,2 0 Wavelength [nm] Absorbance K106 (c = 2.0x10 -5 M) ctDNA (0-8.57x10 -6 M) 450 400 350 300 250 1 0,8 0,6 0,4 0,2 0 Wavelength [nm] Absorbance K107 (c = 2,5x10 -5 M) ctDNA (0 - 5.67x10 -5 M) 0,65 0,73 0,81 0,89 0 2 4 6 8 10 [DNA] (x10 -6 M) Absorbance 0,32 0,36 0,4 0 10 20 30 40 50 60 [DNA] (x10 -6 M) Absorbance Electronic Supplementary Material (ESI) for Molecular BioSystems. This journal is © The Royal Society of Chemistry 2016