161 161 / ﯾﺎﻓﺘﻪ ﻫﺎي ﻧﻮﯾﻦ در ﻋﻠﻮم زﯾﺴﺘﯽ ﺟﻠﺪ7 ، ﺷﻤﺎره2 ، ﺻﻔﺤﺎت161 اﻟﯽ168 ) 1399 ( اﻧﺘﺸﺎرات داﻧﺸﮕﺎه ﺧﻮارزﻣﯽ Nova Biologica Reperta 7(2): 161-168 (2020) Print ISSN: 2423-6330/Online ISSN: 2476-7115 https://nbr.khu.ac.ir; Kharazmi University Press; DOI: 10.29252/nbr.7.2.161 ﺗﺎﺛﯿﺮ ﮐﻮﺋﺮﺳﺘﯿﻦ ﺑﺮ اﺧﺘﻼل ﺣﺎﻓﻈﻪ و اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ ﻧﺎﺷﯽ از ﻣﺴﻤﻮﻣﯿﺖ ﺑﺎ ﻣﺎﻻﺗﯿﻮن در ﻣﻮش ﻫﺎي ﺻﺤﺮاﺋﯽ ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر ﻧﺪا ﺗﻨﺒﺎﮐﻮﭼﯽ ﻣﻘﺪﻣﯽ 1 ، ﺣﻤﯿﺮا ﺣﺎﺗﻤﯽ ﻧﻌﻤﺘﯽ 1 ، ﻏﻼﻣﺮﺿﺎ دﻫﻘﺎن 1 ، ﺳﯿﺪ ﻣﻬﺪي ﺑﺎﻧﺎن ﺧﺠﺴﺘﻪ 1 و ﺣﺎﺗﻢ اﺣﻤﺪي 2 1 ﮔﺮوه زﯾﺴﺖ ﺷﻨﺎﺳﯽ ﺟﺎﻧﻮري، داﻧﺸﮑﺪ ه ﻋﻠﻮم ﻃﺒﯿﻌﯽ، داﻧﺸﮕﺎه ﺗﺒﺮﯾﺰ، ﺗﺒﺮﯾﺰ، اﯾﺮان ؛ 2 ﮔﺮوه ﻋﻠﻮم ﭘﺎﯾﻪ، داﻧﺸﮕﺎه ﻓﺮﻫﻨﮕﯿﺎن، ﺗﻬﺮان، اﯾﺮان ﻣﺴﺌﻮل ﻣﮑﺎﺗﺒﺎت: ، ﺣﺎﺗﻢ اﺣﻤﺪي،hahmadi@cfu.ac.ir ﭼﮑﯿﺪه. ﻣﺎﻻﺗﯿﻮن ﺟﺰو راﯾﺞ ﺗﺮﯾﻦ ﻧﻮع ﺣﺸﺮه ﮐﺶ ﻫﺎ ي ارﮔﺎﻧﻮﻓﺴﻔﺮه اﺳﺖ ﮐﻪ ﺑﻪ ﻋﻨﻮان ﯾﮑﯽ اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ از ﻣﮑﺎﻧﯿﺴﻢ ﻫﺎي ﻣﻠﮑﻮﻟﯽﯿﺖّ اﺻﻠﯽ در ﺳﻤ اﯾﻦ ﻣﺎده ﺑﺮ اﻧﺪام ﻫﺎي ﺑﺪن ﺷﻨﺎﺧﺘﻪ ﺷﺪه اﺳﺖ. ﮐﻮﺋﺮﺳﺘﯿﻦ ﯾﮑﯽ از ﺗﺮﮐﯿﺒﺎت ﻓﻼوﻧﻮﺋﯿﺪي اﺳﺖ. ﻫﺪف ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ ﺑﺮرﺳﯽ اﺛﺮ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﮐﻮﺋﺮﺳﺘﯿﻦ ﺑﺮ ﺣﺎﻓﻈﻪ ﻓﻀﺎﯾﯽ و ﭘﺎراﻣﺘﺮﻫﺎي اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ ﻣﻮش ﻫﺎي ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر ﻃﯽ ﻣﺴﻤﻮ ﻣ ﯿﺖ ﺑﺎ ﺳﻢ ﻣﺎﻻﺗﯿﻮن اﺳﺖ. اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑ ﺮ روي9 ﮔﺮوه8 ﺗﺎﯾﯽ ﻣﻮش ﻫﺎي ﺻﺤﺮاﯾﯽ ﻧﺮ ﺻﻮرت ﮔﺮﻓﺘﻪ اﺳﺖ. ﺳﻪ روز ﭘﺲ از ﺗﺰرﯾﻖ درون ﺻﻔﺎﻗﯽ داروﻫﺎي ﮐﻮﺋﺮﺳﺘﯿﻦ، ﻣﺎﻻﺗﯿﻮن و ﯾﺎ ﺗﺪاﺧﻞ اﯾﻦ داروﻫﺎ ، دﺳﺘﮕﺎه ﻣﺎز آﺑﯽ ﻣﻮرﯾﺲ ﺑﻪ ﻣﻨﻈﻮر ﺳﻨﺠﺶ ﭘﺎراﻣﺘﺮﻫﺎي ﻣﺮﺗﺒﻂ ﺑﺎ ﺣﺎﻓﻈﻪ ﻓﻀﺎﯾﯽ اﺳﺘﻔﺎده ﺷﺪ. ﻧﻤﻮﻧﻪ ﺑﺮداري از ﻫﯿﭙﻮﮐﺎﻣﭗ اﻧﺠﺎم ﮔﺮدﯾﺪ و ﭘﺎ راﻣﺘﺮﻫﺎي اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ در اﯾﻦ ﻧﺎﺣﯿﻪ ﺳﻨﺠﺶ ﮔﺮدﯾﺪ. ﺗﺰرﯾﻖ درون ﺻﻔﺎﻗﯽ دوز ﻫﺎيmg/kg 200 و100 ﻣﺎﻻﺗﯿﻮن ﺑﻪ ﻃﻮر ﻣﻌﻨﯽ داري ﻣﻮﺟﺐ ﮐﺎﻫﺶ ﭘﺎراﻣﺘﺮﻫﺎي ﺣﺎﻓﻈﻪ ﻓﻀﺎﯾﯽ) 01 / 0 < P ( و ﻧﯿﺰ اﻟﻘﺎ اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ ﺷﺪ) 001 / 0 < P .( ﺗﺰرﯾﻖ درون ﺻﻔﺎﻗﯽ دوزmg/kg 50 ﮐﻮﺋﺮﺳﺘﯿﻦ ﺣﺎﻓﻈﻪ ﻓﻀﺎﯾﯽ در رت ﻫﺎي ﻣﺴﻤﻮم ﺷﺪه ﺑﺎ ﻣﺎﻻﺗﯿﻮن را ﺑﻬﺒﻮد ﺑﺨﺸﯿﺪ) 05 / 0 < P .( ﻫﻤﭽﻨﯿﻦ ﭘﺎراﻣﺘﺮﻫﺎي اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ درﮔﺮوه ﻫﺎي ﻣﺴﻤﻮم ﺷﺪه ﺑﺎ ﻣﺎﻻﺗﯿﻮ ﻣﻌﻨﯽ ن، ﺑﺎ ﺗﯿﻤﺎر ﮐﻮﺋﺮﺳﺘﯿﻦ ﮐﺎﻫﺶ داري ﯾﺎﻓﺖ) 01 / 0 < P ( . ﮐﻮﺋﺮﺳﺘﯿﻦ ﻗﺎدر ﺑﻪ ﺑﺎزﮔﺮداﻧﺪن ﻋﻤﻠﮑﺮد ﺣﺎﻓﻈﻪ ﻓ ﻀﺎﯾﯽ و ﻣﻘﺪار ﭘﺎراﻣﺘﺮﻫﺎي اﺳﺘﺮس اﮐﺴﯿﺪا ﺗﯿﻮ ﮔﺮوه ﻫﺎي ﺗﺤﺖ ﺗﯿﻤﺎر ﺑﺎ ﻣﺎﻻﺗﯿﻮن ﺑﻪ ﺳﻄﺢ ﻃﺒﯿﻌﯽ اﺳﺖ. واژه ﻫﺎي ﮐﻠﯿﺪي. ارﮔﺎﻧﻮﻓﺴﻔﺮه، آﻧﺘﯽ اﮐﺴﯿﺪان، ﺗﺮﮐﯿﺒﺎت ﻓﻼﻧﻮﺋﯿﺪي، ﺣﺎﻓﻈﻪ ﻓﻀﺎﯾﯽ، ﻫﯿﭙﻮﮐﺎﻣﭗ The effects of Quercetin on memory and oxidative stress impairment due to Malathion poisoning in male Wistar rats Neda Tanbaccochi Moghadami 1 , Homira Hatami Nemati 1 , Gholamreza Dehghan 1 , Seyyed Mehdi Banan Khojasteh 1 & Hatam Ahmadi 2 1 Department of Animal Biology, Faculty of Natural Sciences, Tabriz University, Tabriz, Iran; 2 Basic Sciences Department, Farhangian University, Tehran, Iran Corresponding Author: Hatam Ahmadi, hahmadi@cfu.ac.ir Abstract. Malathion is one of the commonest type of organophosphate insecticide whose toxicity in human body is mainly considered to result from the induction of oxidative stress. The aim of this study was to investigate the antioxidant effect of Quercetin, a flavonoid compound, on the spatial memory and oxidative stress parameters during Malathion poisoning in male Wistar rats. This study was performed on nine groups, each of which consisted of eight male rats. Three days after intra-peritoneal injection of Quercetin, Malathion or a combination of these two drugs, the Moris Water-Maze apparatus was used to measure spatial memory parameters. The hippocampus was sampled and the oxidative stress parameters were measured in this area. Intra-peritoneal injection of Malathion (100 and 200 mg/kg) significantly reduced spatial memory parameters (P<0.01) and induced oxidative stress (P<0.001), whereas intra- peritoneal injection of quercetin (50 mg/kg) improved spatial memory in Malathion-poisoned experimental rats (P<0.05). Also, oxidative stress parameters in Malathion-treated groups showed a significant reduction in quercetin treatment (P<0.01). Quercetin was observed to restore the function of spatial memory and the level of oxidative stress parameters of the treated groups with Malathion to the normal level. Keywords. antioxidant, hippocampus, flavonoid compounds, organophosphate, spatial memory Received 05.07.2019/ Revised 10.09.2019/ Accepted 16.10.2019/ Published 01.07.2020 درﯾﺎﻓﺖ:14 / 04 / 8 139 / اﺻﻼح:7 0 / 5 0 / 1398 / ﭘﺬﯾﺮش:25 / 6 0 / 1398 / اﻧﺘﺸﺎر:11 / 4 0 / 1399 [ DOI: 10.52547/nbr.7.2.161 ] [ DOR: 20.1001.1.24236330.1399.7.2.10.4 ] [ Downloaded from nbr.khu.ac.ir on 2021-12-07 ] 1 / 8