International Journal of Science and Research (IJSR) ISSN (Online): 2319-7064 Index Copernicus Value (2016): 79.57 | Impact Factor (2017): 7.291 Volume 7 Issue 5, May 2018 www.ijsr.net Licensed Under Creative Commons Attribution CC BY In Vitro Propagation and Sulforaphane Content Analysis Brassica oleraceaevar. Botrytis with TreatmentKinetin and Methionine Wenny S. J. Manoppo 1 , Wenny Tilaar 2 , Dingse Pandiangan 3 1,3 Department of Agronomy Sam Ratulangi University Manado Indonesia, 2 Department of Biology Sam Ratulangi University Manado Indonesia, Jalan Kampus Kleak, Manado, Indonesia, 95115 Abstract: Brassica oleraceae var. Botrytis is a vegetable plant that is quite popular in Indonesia which has high economic value because it contains sulforaphane which has benefits to prevent cancer. This research aims to obtain the best concentration of kinetin and methionine in in vitro culture Brassica oleraceae var. Botrytis,and obtain the best concentration of kinetin and methionine for enhancement of sulforaphane from in vitro cultureBrassica oleraceae var. Botrytis. The results showed that the concentration to obtain the best amount of shoot and leaf number was in the treatment of 5 mg/L kinetin without methionine, while the concentration to obtain the best plant height on the treatment of 100 mg/L methionine without kinetin, and the best plant fresh weight was at treatment combination of 10 mg/L kinetin and 50 mg/L methionine. The plant fresh weight of Brassica oleraceae var. Botrytis is negatively correlated with sulforaphane content in it. The treatment of 50 mg/L of methionine without kinetin was the best treatment in producing a high sulforaphane content of 47.7 ng/g wet weight. Keywords: sulforaphane, kinetin, methionine, Brassica oleraceae var. Botrytis, culture in vitro 1. Introduction White flower cabbage has an important role for human health, because it contains vitamins and minerals are needed by the body, so the demand for vegetables is increasing. Cabbage is included in cabbage or Brassicaceae. Brassicaceae vegetables contain many types of compounds sianohidroksibutena and sulforaphane. Sulforaphane serves to prevent cancer. In addition, this class of vegetables contain high vitamin C which can function as an antioxidant [1]. Sulforaphane is formed from methionine and glucoraphanin which is a precursor or precursor to sulforaphane [2]. The initial precursor in the synthesis of sulforaphane from cruciferaceae is methionine. The final precursor according to the synthetic line of the sulforaphane substance is glucoraphanin [3]. This can increase the production of sulforaphane. In vitro propagation (in vitro culture) has a broad sense that is a method to isolate parts of plants such as protoplasm, cells, a group of cells, tissues, and organs, and grow it in aseptic conditions, so that parts can multiply and generation in to complete plant again [4]. The use of this method has the advantage of being able to produce a culture free of contamination because it is carried out under controlled environmental conditions and is independent of the climate and soil conditions. Another advantage is that cells can be easily propagated to produce specific metabolites [5]. Kinetin is a class of cytokinin hormones. The main function of cytokinin is to stimulate cell division and organ formation. Some of these proteins can be the enzymes needed in mitosis [6]. Cytokines are often added kinetin, zeatin and benzilaminopurin (BAP). Kinetin and BAP are resistant to degradation and are cheaper. In this study used kinetin asgrowth regulator substances of cytokines [7]. The results of Tilaaret al, (1990) [17]. showed that the growing hypocotyl response and broccoli shoots collected on the best MS medium in shoot induction was at a concentration of 5 mg/L BAP without NAA and a combination of NAA 0.01 ppm and 2.5 ppm BAP produce 2-7 shoots. Further research results from Tilaaret al. (2012), sulforaphane content in broccoli (Brassica oleraceae L var. Italica) given combination of methionine treatment 0, 50, 100, and 150 mg/L with broccoli seed extract 0, 1, 2, and 3 gram, the highest was in combination treatment of 100 mg/L methionine and 1 gram of broccoli seed extract that is 182.09 ng/g, followed by treatment of 100 mg/L of methionine without broccoli seed extract of 162.89 ng/g. The highest sulforaphane content in the treatment of 100 mg/L methionine. Based on this research, this research is done by combining it in one stage that is treatment with combination of growth regulator kinetin and amino acid metionin which is sulforafan precursor,using the White Flower Cabbage (Brassica oleraceae var. Botrytis), aimsto obtain the best concentration of Kinetin and Methionine in the formation of shoots from in vitroculture of white flower cabbage (Brassica oleraceae var. Botrytis), and to get the best concentration from Kinetin and Methionine to increase sulforaphane from in vitroculture Flower Cabbage White (Brassica oleraceae var. Botrytis). 2. Research Methods Paper ID: ART20182694 DOI: 10.21275/ART20182694 1483