Rakesh S Shivatare et al., Int. J. Res. Pharm. Sci., 2020, 11(2), 1394-1399 ORIGINAL ARTICLE INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACEUTICAL SCIENCES Published by JK Welfare & Pharmascope Foundation Journal Home Page: www.pharmascope.org/ijrps Isolation, Identiϑication and Characterization of Ximenynic Acid with Anti-Aging Activity from Santalum Album Rakesh S Shivatare *1 , Ramesh Musale 1 , Priya Lohakare 1 , Dipika Patil 1 , Durga Choudhary 2 , Gayatri Ganu 2 , Dheeraj H Nagore 3 , Shailesh M Kewatkar 4 1 JJT University, Jhunjhunu, Rajasthan, India 2 Mprex Healthcare, Pune, India 3 Department of Research and Development, Mprex healthcare, Pune, India 4 Rajarshi Shahu College of Pharmacy, Buldana, Maharashtra, India Article History: Received on: 02.08.2019 Revised on: 08.11.2019 Accepted on: 15.11.2019 Keywords: Ximenynic acid, Santalum album, isolation, Characterization, Antiaging assay ABSTRACT Medicinal plants and its products have been used as a remedial agent in most irising countries for treating diseases. Furthermore, an increasing reliance on the use of medicinal plants in industrialized societies has been traced for the extraction and development of several drugs and chemotherapeutics from these herbal plants. Novel acetylenic fatty acids named Ximenynic acid (XMA) were successfully isolated from the seeds of Santalum album L by N-Hexane extraction. Ximenynic acid (or Santalbic acid) is one of the few acetylenic fatty acids occurring at higher levels in plant seed oils. Ximenynic acid predomi- nantly exists in the seed oil of Santalaceae, Olacaceae, and Opiliaceae families. The structure of XMA was characterized by UV-visible spectroscopy, Infrared Spectrum (IR), NMR Spectroscopy, Differential scanning calorimetry (DSC), Thermal Gravimetric Analysis (TGA), X-ray diffraction (XRD), Fourier Trans- form Infrared Spectroscopy (FT-IR), LCMS spectral analysis. The antiaging activities were assessed by anti-collagenase enzyme assay. Structural analysis revealed that XMA was a crystalline material with a melting point of 38.25 ◦ C and an average molecular weight of 278 kDa. Which is composed of carboxylic acid, butylenic acid, methylene, allylic in their structure. The antiaging assay showed that XMA exhibited signiϑicant collagenase inhibition activity as com- pared with Catechin. These ϑindings suggested that the acetylenic fatty acids XMA could be served as a novel antiaging in Pharmaceutical as well as the cos- metic industry. * Corresponding Author Name: Rakesh S Shivatare Phone: +919890250523 Email: rakeshshivatare@gmail.com ISSN: 0975-7538 DOI: https://doi.org/10.26452/ijrps.v11i2.2005 Production and Hosted by Pharmascope.org © 2020 | All rights reserved. INTRODUCTION Plants are considered an essential source of medicines for humans. Plant parts like leaves, ϑlowers, roots, stems, seeds, and fruit are used as food resources for human as well as a safe medicine for the treatment of different diseases. Plant-derived herbal medicine is used as the main source for the treatment of diseases since ancient times (Hussain et al., 2013) .Ethnopharmacology of natural products isolated from herbal plants possesses pharmacological and therapeutic efϑicacy for treating diseases (Pan et al., 2013). Aging is a natural process in all living organisms. Anti-aging has always been an interest in mankind. After much 1394 © International Journal of Research in Pharmaceutical Sciences