Evaluation of osteopontn, c-telopeptide of type-I collagen and matrix metalloprotein in post- menopausal Egyptian women with osteoporosis. Azza M Abdu Allah 1 , Shereen A. El Tarhouny 2 , Seham A Khodeer 3 , Inass M Taha 4* 1 Department of Biochemistry, Faculty of Medicine, Menofiya University, Egypt 2 Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Taibah University, KSA, Zagazig University, Egypt 3 Clinical Pathology Department, Faculty of Medicine, Menofiya University, Egypt 4 Internal Medicine Department, Faculty of Medicine, Taibah University, Kingdom of Saudi Arabia Abstract Introduction: Osteoporosis is a widespread age-related skeletal disorder characterized by bone loss, which increases skeletal fragility. This study was done to assess the relationship between CTX1, MMP and OPN levels and BMD in postmenopausal Egyptian women. Patient and methods: This search was made on 173 postmenopausal female. MMP-3, OPN and CTX I was done by ELISA. BMD was carried out by dual energy X-ray absorptiometry. According to BMD; they were divided into 3 groups; osteopenia, osteoporosis and control groups. Results: CTX1, OPN and MMP-3 levels were significantly greater in osteoporosis and osteopenia than the control group (P<0.001). In osteoporosis, negative correlations between OPN, calcium and BMD, were found as well as positive correlations between OPN, MMP-3 and CTX-I. By using ROC curve, the sensitivities of both OPN and MMP-3 were equal (93%) and specificities were 100% and 84% respectively. Conclusion: CTX1, OPN and MMP3 were significantly increased in postmenopausal women with osteoporosis suggesting their role in bone turnover. The preserving of normal BMD is a challenge for postmenopausal women to prevent bone disabilities. So, further studies are recommended to use CTX1, OPN and MMP -3 as a regular monitoring system-being sensitive and easy to measure- for early non- invasive detection of osteoporosis in postmenopausal women. Keywords: Osteoporosis, ELISA, Matrix metalloproteinase-3. Accepted on January 10, 2017 Introduction Osteoporosis is a major health problem as dealing with it is very deficient concerning its diagnosis and treatment [1]. Women are more susceptible to osteoporosis than men presenting approximately 80% of all patients with osteoporosis. Generally, women have smaller and thinner bones than men as there is a sharp decline in production of oestrogen after menopause [2]. The method by which decreased level of oestrogen causing bone loss is not clear yet [3]. Bone radiological studies are of little predictive utility, because biochemical changes precede radiological changes. Dual Energy X-Ray Absorptiometry (DEXA) is the standard method to determine bone mineral density. It provides information on changes in bone mineral contents. It is useful for follow up of bone mass or for the study of bone mass changes in the same patient [4]. Matrix Metalloproteinase-3 (MMP-3) is called also stromlysin-1, which is released by osteoblasts. As oestrogen deficiency occurs osteoblast is motivated by osteoclasts. Osteoclasts (OC) resorb bone over a period of 3 weeks, creating cavities that are termed as remodelling space. The resorption is followed by Osteoblast (OB) activation and osteoid formation, filling the cavities over a period of 3 months. So, MMP-3 is released in extra concentrations in matrix around osteocyte and its lacuna in order to take role in collagen and cartilage metabolism [3,5]. In pre-mature bone tissue, non-collagen was altered by OPN/MMP-3 complexity before mineralization of bone matrix so as to keep local micro circumstance by protein-lyses of complexity. It occurs in osteoporosis enhanced by osteoclasts As osteoblasts is creeping and persisting in states with increasing mineralization and high bone turn-over [6,7]. Approximately 90% of the organic matrix of bone is type I collagen, a helical protein that is cross linked at the N- and C- ISSN 0970-938X www.biomedres.info 3645 Biomedical Research 2017; 28 (8): 3645-3650 Biomed Res- India 2017 Volume 28 Issue 8