1 Mahasiswa Jurusan Teknologi Pertanian, Fakultas Pertanian, Universitas Riau 2 Staf Pengajar Jurusan Teknologi Pertanian, Fakultas Pertanian, Universitas Riau JOM FAPERTA VOL. 4 NO. 2 OKTOBER 2017 1 AKTIVITAS ANTIMIKROBA Lactobacillus plantarum 1 YANG DIISOLASI DARI INDUSTRI PENGOLAHAN PATI SAGU TERHADAP BAKTERI PATOGEN Escherichia coli FNCC-19 DAN Staphylococcus aureus FNCC-15 ANTIMICROBIAL ACTIVITY of Lactobacillus plantarum 1 ISOLATED FROM SAGO STARCH PROCESSING INDUSTRY AGAINST BACTERIAL PATHOGENS Escherichia coli FNCC-19 AND Staphylococcus aureus FNCC-15 Noni Afriani 1 , Yusmarini 2 and Usman Pato 2 Program Studi Teknologi Hasil Pertanian, Jurusan Teknologi Pertanian, Fakultas Pertanian, Universitas Riau, Kode Pos 28293, Indonesia Noniafrianithp@yahoo.com ABSTRACT The purpose of this study was to determined and measure the antimicrobial activity of Lactobacillus plantarum 1 isolated from sago starch processing industry against pathogenic bacteria Escherichia coli FNCC-15 and Staphylococcus aureus FNCC-19. The research was carried out experimentally using Completely Randomized Design (CRD) with four as treatments and four replications. Antimicrobial activity was tested using the well diffusion method and the paper disc diffusion method. Result of analysis of variance showed that the antimicrobial activity of lactic acid bacteria isolates and cell-free supernatant of bacteria Staphylococcus aureus FNCC-15 and Escherichia coli FNCC-19 were significantly different (P<0.05%). T test result showed that the inhibition zone produced acid between bacteria isolates to cell-free supernatant were significantly different (P<0.05%). The average diameter inhibition zone of lactic acid bacteria isolated against Staphylococcus aureus FNCC-15 were from 6.10 to 8.08 mm. The average diameter inhibition zone of lactic acid bacteria isolated against Escherichia coli FNCC-19 were from 6.80 to 9.20 mm. The average diameter cell-free supernatant inhibition zone against bacteria Staphylococcus aureus FNCC-15 were from 1.22 to 2.41 mm and inhibition cell-free supernatant against Escherichia coli FNCC-19 ranged from 2.06 to 3.50 mm. The results of this study indicate that the isolates of Lactobacillus plantarum 1 RN2-12112 and the supernatant had greater antimicrobial activity than isolates of Lactobacillus plantarum 1RN2-53. Keywords: Lactic acid, Antimicrobials, Lactobacillus plantarum 1, Supernatant. PENDAHULUAN Senyawa antimikroba adalah senyawa kimiawi atau biologis yang dapat menghambat pertumbuhan dan aktivitas mikroba, baik secara alamiah ataupun secara sengaja ditambahkan ke dalam makanan. Bakteri asam laktat (BAL) dapat menghasilkan senyawa antimikroba yang mampu menghambat