220 RESEARCH/ INVESTIGACIÓN DETECTION OF BURSAPHELENCHUS MUCRONATUS ASSOCIATED WITH ACANTHOCINUS GRISEUS ON PINUS NIGRA IN THE NORTHWESTERN TURKEY A. Tülek 1* , I. Kepenekci 2 , M. İmren 3 , S. Akbulut 4 , B. Tülek 1 , A.S. Koca 3 , and H. Özdikmen 5 1 Trakya Agricultural Research Institute, Edirne, Turkey; 2 Department of Plant Protection, Faculty of Agriculture, Gaziosmanpaşa University, Tokat, Turkey; 3 Department of Plant Protection, Faculty of Agriculture and Natural Sciences, Abant Izzet Baysal University, Bolu, Turkey; 4 Department of Forest Engineering, Faculty of Forestry, Katip Çelebi University, İzmir, Turkey; 5 Department of Biology, Faculty of Science, Gazi University, Ankara, Turkey; *Corresponding author: adnantulek@gmail.com ABSTRACT Tülek, A., I. Kepenekci, M. İmren, S. Akbulut, B. Tülek, A.S. Koca, and H. Özdikmen. 2019. Detection of Bursaphelenchus mucronatus associated with Acanthocinus griseus on Pinus nigra in the northwestern Turkey. Nematropica 49:220-228. A survey was conducted in Edirne Province, Turkey for the pinewood nematode, Bursaphelenchus xylophilus. Wood samples were collected from declining black pine trees. Nematodes were extracted from wood samples and insect specimens were collected. Nematodes were observed and identified microscopically and molecularly with the internal transcribed spacer (ITS) of ribosomal DNA. Bursaphelenchus xylophilus was not detected, but B. mucronatus was found in very high numbers with an average up to 300 nematodes/g of wood shaving. Acanthocinus griseus (Coleoptera: Cerambycidae) was detected to be associated with B. mucronatus as a potential vector. This is the first report of B. mucronatus association with A. griseus in Turkey. Key words: Acanthocinus griseus, black pine, Bursaphelenchus mucronatus, insect vector, pine wilt disease RESUMEN Tülek, A., I. Kepenekci, M. İmren, S. Akbulut, B. Tülek, A.S. Koca, and H. Özdikmen. 2019. Detección de Bursaphelenchus mucronatus asociada con Acanthocinus griseus en Pinus nigra en el noroeste de Turquía. Nematropica 49:220-228. Se realizó una encuesta en la provincia de Edirne ubicada en la parte noroeste de Turquía para el nematodo de madera de pino. Se tomaron muestras de madera de pinos en declive. Se extrajeron nematodos de muestras de madera y se recolectaron muestras de insectos. Se observaron e identificaron nematodos. No se detectó Bursaphelenchus xylophilus, pero se encontraron especies de B. mucronatus en cantidades muy altas, con un promedio de hasta 300 nematodos por 1 g de viruta de madera, obtenida de pinos negros. Acanthocinus griseus (Coleoptera: Cerambycidae) se detectó como un vector de B. mucronatus. Este es el primer informe de la asociación entre B. mucronatus y A. griseus de Turquía.