1 SUPPLEMENTARY MATERIAL: The Wnt-5a-derived hexapeptide Foxy-5 inhibits breast cancer metastasis in vivo by targeting cell motility Annette Säfholm, Johanna Tuomela, Jeanette Rosenkvist, Janna Dejmek, Pirkko Härkönen and Tommy Andersson* Supplementary Methods: Reverse transcription-polymerase chain reaction (RT-PCR) Total mRNA from T47D, MDA-MB-468 and 4T1 cells was obtained using TRIzol ® Reagent according to the manufacturer's protocol. Isolated total RNA was reversed transcribed using Superscript™ II RNase H-reverse transcriptase (Invitrogen, Carlsbad, CA), per the manufacturer's instructions. To control for DNA contamination, the reverse transcription reaction was performed without the reverse transcriptase enzyme. The RT reaction products and 1μg of control genomic DNA extracted from the 4T1 cells was then subjected to PCR using Taq DNA polymerase (Invitrogen, Carlsbad, CA). The following primer sets were used for PCR: -actin F (5'-TTC AAC ACC CCA GCC ATG TA-3'), -actin R (5'-TTG CCA ATG GTG ATG ACC TG-3'), corresponding to nucleotides 452-471 and 809-828 of the human -actin sequence; human Ror2 F (5'-CCC