Tis article is protected by copyright. To share or copy this article, please visit copyright.com. Use ISSN#1078-6791. To subscribe, visit alternative-therapies.com Chavan—Hepatoprotective Activity of Satwa Against Alcohol Injury 34 ALTERNATIVE THERAPIES, JUL/AUG 2017 VOL. 23 NO. 4 Hepatoprotective Activity of Satwa, an Ayurvedic Formulation, Against Alcohol-induced Liver Injury in Rats Tejaswi Chavan, MSc; Abhijit Ghadge, MSc; Manjiri Karandikar, MD; Vijaya Pandit, MD; Prabhakar Ranjekar, PhD; Omkar Kulkarni, MD; Aniket Kuvalekar, PhD; Nitin Mantri, PhD ORIGINAL RESEARCH ABSTRACT Context • Guduchi Satwa is an Ayurvedic formulation prepared from Tinospora species. It has been used since ancient times to treat liver disorders. Objectives • Te study intended to assess the hepatoprotective potential of Satwa prepared from 3 forms of Tinospora against alcohol-induced hepatotoxicity. Design • Male, albino Wistar rats were divided into 6 groups, with 6 rats each: 3 control groups—healthy controls, negative controls, and positive controls—and 3 intervention groups—Tinospora cordifolia, Tinospora sinensis, and Neem-Guduchi. Setting • Te study was carried out at the Animal House facility of Bharati Vidyapeeth Deemed University’s Medical College (Maharashtra, India). Intervention • Hepatotoxicity was induced by repeated dosing with alcohol for 15 d for all groups except for the healthy controls. To induce hepatotoxicity, the 5 groups received 1 mL of 30% alcohol PO per 100 g of body weight per day. Te healthy controls and the negative controls received no hepatoprotective treatments. Te other 4 groups received the dosing with alcohol 30 min afer the hepatoprotective treatment, which they also received for 15 d: (1) positive controls—100 mg of silymarin per kg of body weight per day PO; (2) intervention group 1 (T cordifolia group)—200 mg of T cordifolia per kg of body weight per day PO; (3) intervention group 2 (T sinensis group)—200 mg of T sinensis per kg of body weight per day PO; and (4) intervention group 3 (Neem-Guduchi group)—200 mg of Neem-Guduchi per kg of body weight per day PO. Outcome Measures • Serum and liver tissue were used for biochemical analysis. Results • For the negative and positive control groups and the 3 intervention groups, the repeated dosing with alcohol produced elevations in the levels of liver-marker enzymes and changes in the lipid-profle status of the animals. Satwa from T cordifolia had a specifc action in maintaining the lipid profile: total cholesterol, high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein. Improvement in the hepatic function, normalization of the lipid profle in the serum and liver, and improvements in the levels of antioxidant enzymes and oxidative-stress markers were observed in the animals treated with T sinensis Satwa. Neem-Guduchi Satwa was found to have a specifc action in maintaining the lipid profle. Te diferential hepatoprotective efect of that Satwa was also evident from the liver histology. Conclusions • Te data suggest that the 3 Satwa might be used in combination as a liver tonic that can help restore and strengthen the liver functions. Te current study shows that the combination has the potential to be an efective liver tonic in animals. Scientifc data from clinical trials of the 3 Satwa are not available. Systematic clinical trials are required that can yield information on their efects in humans. (Altern Ter Health Med. 2017;23(4):34-40) Tejaswi Chavan, MSc, is a Phd scholar at the Interactive Research School for Health Afairs (IRSHA), Bharati Vidyapeeth Deemed University (BVDU), in Maharashtra, India; Abhijit Ghadge, MSc, is a Phd scholar at IRSHA; Manjiri Karandikar, MD, is a professor in the Department of Pathology, Bharati Vidyapeeth Medical College (BVMC), BVDU, in Maharashtra, India; Vijaya Pandit, MD, is professor and head of the Department of Pharmacology, BVMC, BVDU, in Maharashtra, India; Prabhakar Ranjekar, PhD, is the former director at IRSHA; Omkar Kulkarni, MD, is head of pharma research and business development at the Vishwanand Kendra Center for Integrative Medicine and Research, in Maharashtra, India; Aniket Kuvalekar, PhD, is an assistant professor at IRSHA; Nitin Mantri, PhD, is a senior lecturer at the School of Science, RMIT University, in Melbourne, Australia. Corresponding author: Nitin Mantri, PhD E-mail address: nitin.mantri@rmit.edu.au